Transient Intermediates of Chemical and Biochemical Reactions

化学和生化反应的瞬时中间体

• 批准号: 0349595
• 负责人: Frantisek Turecek
• 金额: $ 55万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0349595&HistoricalAwards=false
• 关键词: Transient; Intermediates; Chemical; Biochemical; Reactions

With this renewal proposal the Organic and Macromolecular Chemistry Program continues its support for the work of Professor Frantisek Turecek of the Department of Chemistry at the University of Washington in Seattle, WA. The research will continue the PI's research on the chemistry of transient intermediates in reactions of biological interest. These transients will be investigated in the gas phase in the absence of solvent and other complicating factors allowing intrinsic properties to be determined. The experimental observations will be complemented by sophisticated calculation of the structure and energy of these species. Since the intermediates to be studied will be derived from DNA nucleobases, amino acids, and peptides, this research will contribute fundamental data in the area of protein and nucleic acid chemistry.These studies are expected to have broad impact upon the biological sciences. In particular, they will contribute to our knowledge of factors responsible for DNA and protein damage. In addition these experiments will provide a significant base of knowledge in the areas of proteomics and genomics in which many of the recent advances have been achieved through mass spectrometry. The research will also provide valuable training for students in the area of biological mass spectrometry.

通过这项更新提案，有机和高分子化学计划继续支持华盛顿州西雅图的华盛顿大学化学系的Frantisek Turecek教授的工作。 该研究将继续PI对生物学反应中瞬态中间体化学的研究。 这些瞬态将在气相中进行研究，在没有溶剂和其他复杂因素的情况下，允许确定固有特性。 实验观测将通过对这些物种的结构和能量的复杂计算来补充。 由于所研究的中间体将来自DNA碱基、氨基酸和肽，因此本研究将为蛋白质和核酸化学领域提供基础数据，这些研究将对生物科学产生广泛的影响。 特别是，它们将有助于我们了解导致DNA和蛋白质损伤的因素。此外，这些实验将在蛋白质组学和基因组学领域提供重要的知识基础，其中许多最新进展是通过质谱法实现的。该研究还将为生物质谱领域的学生提供有价值的培训。