Protein Adsorption and Transport in Chromatographic Bioprocessing

摘要项目名称：蛋白质在色谱生物处理中的吸附和转运项目编号：cts -0350631项目负责人：Abraham LenhoffInstitution of delaware大学本项目旨在研究蛋白质在色谱分离中的吸附和转运机制。一个目标是发展蛋白质的结构特性和固定相之间的定量关系。色谱性能将以线性保留、容量和颗粒内运输为特征。这些措施与固定相、蛋白质和洗脱液的潜在物理、化学和结构性质有关。研究将集中在两种色谱模式：离子交换色谱（IEC）和疏水相互作用色谱（HIC）。蛋白质吸附动力学也将被确定。就更广泛的影响而言，色谱法广泛应用于生物加工的下游纯化。提出的研究可以提供定量的机制模型，可用于指导分离过程。学生的培训将涵盖从共聚焦显微镜等现代实验工具到生物加工中色谱法的实践。

AbstractProposal Title: Protein Adsorption and Transport in Chromatographic BioprocessingProposal Number: CTS-0350631Principal Investigator: Abraham LenhoffInstitution: University of DelawareThe objective of this project is to determine the mechanisms of adsorption and transport in chromatographic separations of proteins. One goal is to develop quantitative relationships between structural properties of the protein and the stationary phase. Chromatographic performance will be characterized in terms of linear retention, capacity, and intraparticle transport. These measures are related to the underlying physical, chemical and structural properties of the stationary phase, the protein, and the eluent. Research will focus on two modes of chromatography: ion exchange chromatography (IEC) and hydrophobic interaction chromatography (HIC). Protein adsorption kinetics will also be determined. In terms of the broader impacts, chromatography is widely used in downstream purification in bioprocessing. The proposed studies can provide quantitative mechanistic models that can be used to guide separation processes. Student training will span a spectrum from modern experimental tools such as confocal microscopy to the practice of chromatography in bioprocessing.