Molecular Biophysics of Protein Adsorption in Ion-Exchange Chromatography

离子交换色谱中蛋白质吸附的分子生物物理学

• 批准号: 9321318
• 负责人: Abraham Lenhoff
• 金额: $ 24.26万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-15 至 1999-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9321318&HistoricalAwards=false
• 关键词: Molecular; Biophysics; Protein; Adsorption; Ion

The aim of this research is the development of molecular thermodynamic models to predict protein adsorption in ion exchange chromatography. Models will incorporate electrostatic and van der Waals components for protein-surface and protein-interactions with particular interests related to adsorption patterns and molecular orientations. Brownian dynamics simulations are to be performed in order to examine kinetic effects. A complementary experimental component comrises characterization of protein adsorption on quartz or silica by total internal reflectance fluorescence (TIRF) spectroscopy, surface force measurements, atomic force microscopy and other methods. This project should help improve one of the major methods for purification of some pharmaceuticals and products of genetic engineering technologies.

本研究的目的是发展分子热力学模型来预测蛋白质在离子交换层析中的吸附。 模型将结合静电和货车的蛋白质表面和蛋白质的相互作用与有关的吸附模式和分子取向的特殊利益的组成部分。 为了研究动力学效应，将进行布朗动力学模拟。 补充实验部分包括通过全内反射荧光（TIRF）光谱、表面力测量、原子力显微镜和其他方法表征蛋白质在石英或二氧化硅上的吸附。 该项目应有助于改进某些药物和基因工程技术产品的主要提纯方法之一。