The Thermodynamics of Protein Separations

9510420 Lenhoff该提案是关于研究继续开发用于蛋白质分离的分析和设计的定量热力学基础，与常规应用于化学加工工业中的常规分离的热力学基础相当。 这将涉及以下方面的研究：（1）基于蛋白质结构（当已知时）和基于关键实验生物物理特征（当结构未知时）预测溶液中蛋白质的关键热力学性质的分子理论，以及（2）使用相行为和物理性质的描述来开发合理的、基于热力学的方法来预测重要过程的分离性能，包括沉淀和两相水萃取。 ***

9510420 Lenhoff This proposal is on research to continue to develop a quantitative thermodynamic basis for the analysis and design of protein separations, comparable to that which is routinely applied to conventional separations in the chemical process industries. This will involve research on: (1) molecular theories to predict key thermodynamic properties of proteins in solution, based on protein structure, when it is known, and on key experimental biophysical characteristics, when the structure is not known, and (2) use of the descriptions of phase behavior and physical properties to develop rational, thermodynamically- based approaches to predict separation performance for important processes, including precipitation and two-phase aqueous extraction. ***