Aberration-Corrected Photoelectron Microscope: Opening the Nanometer Scale for Organic Matter Microscopy

像差校正光电子显微镜：打开有机物显微镜的纳米尺度

• 批准号: 0352224
• 负责人: Rolf Koenenkamp
• 金额: $ 74.1万
• 依托单位: Portland State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-15 至 2008-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0352224&HistoricalAwards=false
• 关键词: Aberration; Corrected; Photoelectron; Microscope; Opening

This award supports the development of a stand-alone aberration-corrected photoelectron microscope (PEM), capable of 2 nm spatial resolution. Photoelectron microscopes combine the advantages of sample exposure by light and the high-resolution capabilities of imaging with electrons. These unique features are of particular interest in biology since they allow the damage-free study of soft organic matter at very high resolution. The resolution of the proposed microscope will be comparable to the best attainable with scanning electron microscopes. Yet the ultraviolet (uv)-excitation of photoelectron microscopes is considerably less damaging than the electron exposure in scanning electron microscopes, particularly when a narrow electron beam is used as in high-resolution work. Photoelectron microscopy also allows a number of imaging modes, which are not accessible with scanning electron microscopes. These imaging modes include specific chemical, physical, and topographical contrast modes as well as advanced operation with incorporated markers. These features can be used to obtain strong functional contrast in tissues of similar atomic composition. At present no other light-optical excitation technique is capable of better resolution. The physical design of the instrument will incorporate a hyperbolic electrostatic mirror to correct the spherical and chromatic aberrations of an electrostatic lens system. A Y-shaped beam line with symmetric small-angle deflections will allow a compact design, compatible with typical laboratory dimensions. This work will bring together an interdisciplinary team of engineers, technicians, and scientists from within the university and from industry. Students at all levels (high-school, undergraduate, graduate, postgraduate) will be involved in and/or exposed to the technology development effort.

该奖项支持开发独立的像差校正光电子显微镜(PEM)，能够实现2纳米的空间分辨率。光电子显微镜结合了样品光曝光的优点和电子成像的高分辨率能力。这些独特的特征在生物学中特别令人感兴趣，因为它们允许以非常高的分辨率对软有机物进行无损研究。所提出的显微镜的分辨率将可与扫描电子显微镜所能达到的最佳分辨率相媲美。然而，光电子显微镜的紫外线(UV)激发比扫描电子显微镜的电子曝光危害小得多，特别是在高分辨率工作中使用窄电子束的情况下。光电子显微镜还允许许多扫描电子显微镜无法访问的成像模式。这些成像模式包括特定的化学、物理和地形对比模式，以及结合标记的高级操作。这些特征可以用来在原子组成相似的组织中获得强烈的功能对比。目前，还没有其他的光-光激发技术能够更好地分辨。该仪器的物理设计将包括一个双曲静电镜，以校正静电透镜系统的球差和色差。具有对称小角偏转的Y形光束线将允许紧凑的设计，与典型的实验室尺寸兼容。这项工作将汇聚一支由来自大学内部和行业的工程师、技术人员和科学家组成的跨学科团队。所有级别的学生(高中、本科生、研究生、研究生)都将参与和/或接触到技术开发工作。