Development of Far-Infrared Difference Spectroscopy For Studies of Biological Pigment-Protein Complexes

用于生物色素-蛋白质复合物研究的远红外差分光谱技术的发展

• 批准号: 0352324
• 负责人: Gary Hastings
• 金额: --
• 依托单位: Georgia State University Research Foundation, Inc.
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0352324&HistoricalAwards=false
• 关键词: Development; Far; Infrared; Difference; Spectroscopy

This award supports the development of an instrument for Fourier transform infrared (FTIR) difference spectroscopic measurement of biological pigment-protein complexes in the 1000-140 cm-1 spectral region. At present few instruments can perform measurements on biological samples below ~1000 cm-1. Instruments that do have sensitivity below ~1000 cm-1 are inherently slow, and measurements typically extend over several days. Here, an instrument will be constructed with sensitivity down to ~140 cm-1, with data acquisition rates at least two orders of magnitude superior to existing instruments. Such an instrument will make far-IR difference measurements of biological samples simpler. The main advance is in the fabrication, testing and incorporation of new fast and sensitive far-infrared detectors into our FTIR instrumentation. Extension of FTIR difference techniques down to ~140 cm-1 is highly desirable as it will open up a window where the bonds between metals and organic ligands (Mn.O, Mg.N, Fe.N, Fe.S), characteristic of a large number of the protein cofactors can be directly investigated.

该奖项支持在1000-140 cm-1光谱区域内开发用于生物色素-蛋白质复合物的傅里叶变换红外（FTIR）差分光谱测量的仪器。目前，很少有仪器可以对低于1000 cm-1的生物样品进行测量。灵敏度低于~1000 cm-1的仪器本身就很慢，测量通常要持续几天。 在这里，仪器将被构造成具有低至~140 cm-1的灵敏度，数据采集速率比现有仪器至少高上级两个数量级。 这种仪器将使生物样品的远红外差异测量更简单。主要的进展是在制造，测试和纳入新的快速和敏感的远红外探测器到我们的FTIR仪器。 将FTIR差示技术扩展到~140 cm-1是非常可取的，因为它将打开一个窗口，其中可以直接研究金属和有机配体（Mn.O、Mg.N、Fe.N、Fe.S）之间的键，这些键是大量蛋白质辅因子的特征。