High Resolution Laser Speckle Strain Gauge for Biomedical Research and Diagnostics

用于生物医学研究和诊断的高分辨率激光散斑应变计

• 批准号: 0352608
• 负责人: Sean Kirkpatrick
• 金额: $ 19.21万
• 依托单位: Oregon Health & Science University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0352608&HistoricalAwards=false
• 关键词: Resolution; Laser; Speckle; Strain; Gauge

0201841KirkpatrickThe proposal focused on continued development and implementation of a high resolution, imaging laser speckle-based strain gauge for biomedical research and medical diagnostic purposes. During the period of this award, the investigators propose to expand the useful range of the system to the level of single cells and single-cell thick cell layers and to develop and implement an optical means by which the viscoelastic properties of tissues and cell sheets can be measured directly in a nondestructive manner. The hypotheses driving this research are: 1) Improved laser speckle strain measurement technology can provide detailed information on the mechanical behavior of tissues at the cellular level with better strain resolution than strain technologies conventionally employed for these purposes, including classical interferometric technologies, 2) Variations of the speckle strain gauge can yield information regarding the viscoelastic constants of biological tissues such as mechanical loss factor (tan d), storage modulus, and loss modulus and 3) The strain gauge technology can be modified to provide full 2D strain data of tissues under biaxial loading. The specific aims to test these hypotheses are: 1) To implement an imaging laser speckle strain gauge for evaluating the micromechanical behavior of single-cells and cell sheets, 2) To develop a variation of the speckle strain gauge to yield viscoelastic constants such as loss factor (tan d), storage modulus and loss modulus and 3) To modify the speckle strain gauge to acquire and analyze 2-dimensional strain data. Results of the studies should have a significant impact on a variety of modern diagnostic and therapeutic applications.

该提案的重点是继续开发和实施用于生物医学研究和医学诊断目的的高分辨率成像激光散斑应变计。在此期间，研究人员建议将该系统的使用范围扩展到单细胞和单细胞厚细胞层的水平，并开发和实施一种光学手段，通过这种手段，组织和细胞片的粘弹性特性可以以非破坏性的方式直接测量。推动这项研究的假设是：1)改进的激光散斑应变测量技术可以在细胞水平上提供组织力学行为的详细信息，其应变分辨率优于传统的应变技术，包括经典的干涉测量技术。2)散斑应变测量仪的变化可以产生有关生物组织的粘弹性常数的信息，如机械损失因子（tan d），存储模量，3)对应变片技术进行改进，可提供组织在双轴载荷下的完整二维应变数据。验证这些假设的具体目标是：1)实现用于评估单细胞和细胞片微观力学行为的成像激光散斑应变仪；2)开发散斑应变仪的变体以获得粘弹性常数，如损耗因子（tan d）、存储模量和损耗模量；3)修改散斑应变仪以获取和分析二维应变数据。研究结果将对各种现代诊断和治疗应用产生重大影响。