A Capillary DNA Sequencer, Quantitative RT-PCR Machine, and Digital Phosphor/Fluorescent Imager for a Multi-User Facility in the University of Oklahoma Department of Zoology

俄克拉荷马大学动物系多用户设施的毛细管 DNA 测序仪、定量 RT-PCR 机和数字荧光/荧光成像仪

• 批准号: 0400791
• 负责人: Richard Broughton
• 金额: $ 17.99万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-15 至 2006-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0400791&HistoricalAwards=false
• 关键词: Capillary; DNA; Sequencer; Quantitative; RT

This award provides partial support for the purchase of three major equipment items: a 16-capillary DNA sequencer, a real-time quantitative PCR machine, and a digital phosphor/fluorescent imager. All three of these items will enhance an existing multi-user core facility for molecular biology research in the Department of Zoology at the University of Oklahoma-Norman Campus. The use of molecular methods has become essential in nearly all areas of biology. Beyond being central to molecular genetics, cell, and developmental biology, there has been rapid growth in the use of molecular techniques in areas such as ecology, systematics, evolution, and behavior. Molecular biology itself has recently been driven by large-scale genome sequencing and proteomics projects, with emergent new technologies and associated instruments. Studies in nearly all areas of biology increasingly focus on analysis of DNA sequence, DNA fragment (e.g., single nucleotide polymorphisms [SNPs] and microsatellites), and gene expression data, requiring access to sophisticated equipment frequently beyond the resources of individual investigators or labs. The instrumentation will also enhance undergraduate, graduate, and post-graduate research training opportunities at the University of Oklahoma.

该合同为购买三个主要设备项目提供部分支持：16毛细管DNA测序仪，实时定量PCR机和数字荧光粉/荧光成像仪。这三个项目都将加强俄克拉何马大学诺曼校区动物学系现有的多用户分子生物学研究核心设施。分子方法的使用在生物学的几乎所有领域都是必不可少的。除了作为分子遗传学、细胞生物学和发育生物学的核心之外，分子技术在生态学、系统学、进化和行为学等领域的应用也在迅速增长。分子生物学本身最近受到大规模基因组测序和蛋白质组学项目的推动，伴随着新兴的新技术和相关仪器。几乎所有生物学领域的研究都越来越关注DNA序列、DNA片段（例如，单核苷酸多态性[SNPs]和微卫星）和基因表达数据的分析，这需要使用复杂的设备，而这些设备往往超出了单个研究人员或实验室的资源。该仪器还将增加俄克拉何马大学本科生、研究生和研究生的研究培训机会。