A Capillary DNA Sequencer, Quantitative RT-PCR Machine, and Digital Phosphor/Fluorescent Imager for a Multi-User Facility in the University of Oklahoma Department of Zoology

该合同为购买三种主要设备提供部分支持：一台16毛细管DNA测序仪、一台实时定量聚合酶链式反应机器和一台数字磷光/荧光成像仪。所有这三个项目都将加强俄克拉荷马大学诺曼校区动物系现有的多用户核心分子生物学研究设施。分子方法的使用在生物学的几乎所有领域都变得必不可少。除了作为分子遗传学、细胞和发育生物学的中心，分子技术在生态学、系统学、进化论和行为学等领域的应用也迅速增长。分子生物学本身最近受到大规模基因组测序和蛋白质组学项目的推动，新技术和相关仪器不断涌现。生物学几乎所有领域的研究都越来越侧重于DNA序列、DNA片段(例如单核苷酸多态[SNPs]和微卫星)和基因表达数据的分析，这就需要经常使用超出个人研究人员或实验室资源的尖端设备。该仪器还将增加俄克拉荷马大学的本科生、研究生和研究生的研究培训机会。

This award provides partial support for the purchase of three major equipment items: a 16-capillary DNA sequencer, a real-time quantitative PCR machine, and a digital phosphor/fluorescent imager. All three of these items will enhance an existing multi-user core facility for molecular biology research in the Department of Zoology at the University of Oklahoma-Norman Campus. The use of molecular methods has become essential in nearly all areas of biology. Beyond being central to molecular genetics, cell, and developmental biology, there has been rapid growth in the use of molecular techniques in areas such as ecology, systematics, evolution, and behavior. Molecular biology itself has recently been driven by large-scale genome sequencing and proteomics projects, with emergent new technologies and associated instruments. Studies in nearly all areas of biology increasingly focus on analysis of DNA sequence, DNA fragment (e.g., single nucleotide polymorphisms [SNPs] and microsatellites), and gene expression data, requiring access to sophisticated equipment frequently beyond the resources of individual investigators or labs. The instrumentation will also enhance undergraduate, graduate, and post-graduate research training opportunities at the University of Oklahoma.