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A Capillary DNA Sequencer, Quantitative RT-PCR Machine, and Digital Phosphor/Fluorescent Imager for a Multi-User Facility in the University of Oklahoma Department of Zoology

A Capillary DNA Sequencer, Quantitative RT-PCR Machine, and Digital Phosphor/Fluorescent Imager for a Multi-User Facility in the University of Oklahoma Department of Zoology
俄克拉荷马大学动物系多用户设施的毛细管 DNA 测序仪、定量 RT-PCR 机和数字荧光/荧光成像仪
批准号:
0400791
负责人:
Richard Broughton
金额:
$17.99万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
2004
资助国家:
美国
项目状态:
已结题
起止时间:
2004-05-15 至 2006-04-30

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中文摘要
翻译
该奖项为三个主要设备项目的购买提供部分支持:16毛细管DNA测序仪,实时定量PCR机和数字荧光/荧光成像仪。 所有这三个项目将加强现有的多用户核心设施的分子生物学研究在动物学系在俄克拉荷马大学诺曼校区。 分子方法的使用在几乎所有生物学领域都变得至关重要。 除了作为分子遗传学、细胞和发育生物学的核心之外,分子技术在生态学、系统学、进化和行为等领域的应用也在迅速增长。 分子生物学本身最近受到大规模基因组测序和蛋白质组学项目的推动,出现了新技术和相关仪器。 几乎所有生物学领域的研究越来越多地集中在DNA序列、DNA片段(例如,单核苷酸多态性[SNPs]和微卫星)和基因表达数据,需要访问复杂的设备,往往超出了个人研究者或实验室的资源。 仪器还将提高本科生,研究生和研究生的研究培训机会,在俄克拉荷马州大学。
英文摘要
This award provides partial support for the purchase of three major equipment items: a 16-capillary DNA sequencer, a real-time quantitative PCR machine, and a digital phosphor/fluorescent imager. All three of these items will enhance an existing multi-user core facility for molecular biology research in the Department of Zoology at the University of Oklahoma-Norman Campus. The use of molecular methods has become essential in nearly all areas of biology. Beyond being central to molecular genetics, cell, and developmental biology, there has been rapid growth in the use of molecular techniques in areas such as ecology, systematics, evolution, and behavior. Molecular biology itself has recently been driven by large-scale genome sequencing and proteomics projects, with emergent new technologies and associated instruments. Studies in nearly all areas of biology increasingly focus on analysis of DNA sequence, DNA fragment (e.g., single nucleotide polymorphisms [SNPs] and microsatellites), and gene expression data, requiring access to sophisticated equipment frequently beyond the resources of individual investigators or labs. The instrumentation will also enhance undergraduate, graduate, and post-graduate research training opportunities at the University of Oklahoma.
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