SGER: Cellular Interrogation Using Fiberoptic Nanobiosensors

SGER：使用光纤纳米生物传感器进行细胞询问

• 批准号: 0406282
• 负责人: Kunal Mitra
• 金额: $ 4.32万
• 依托单位: Florida Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0406282&HistoricalAwards=false
• 关键词: SGER; Cellular; Interrogation; Using; Fiberoptic

0406282MitraThe investigators will study the mechanism of laser therapy using fiberoptic nanobiosensors. Low intensity ultraviolet, visible and near and mid-infrared radiation will be carefully aimed and injected onto and through the cell membrane with tiny evanescent fiber and micropipette-like nanoprobes constructed for efficient delivery of the radiation onto sub-cellular organelles. Microprobes will be used to sense light by photon counting and the emission of chemicals will be sensed by mid-infrared absorption using evanescent tips. Responses could occur in the form of light emission such as luminescence from singlet oxygen or a chemical emission. A major goal is to determine the photochemical mechanism responsible for cell stimulation in the case of laser therapy and cell death in the case of photodynamic therapy.

0406282Mitra研究人员将使用光纤纳米生物传感器研究激光治疗的机制。 低强度的紫外线、可见光、近红外线和中红外线辐射将被仔细瞄准并注入到细胞膜上，并通过微小的渐逝纤维和微移液管样纳米探针将辐射有效地传递到亚细胞器上。 微探针将用于通过光子计数来感测光，并且化学物质的发射将通过使用渐逝尖端的中红外吸收来感测。响应可以以光发射的形式发生，例如来自单线态氧的发光或化学发射。 一个主要的目标是确定负责细胞刺激的情况下，激光治疗和细胞死亡的情况下，光动力治疗的光化学机制。