Acquisition of an integrated scanning probe, scanning laser, optical microscope

0132433 Lower这笔赠款为一台集光学显微镜复制、共聚焦激光扫描显微镜、扫描探针显微镜和光钳为一体的最先进的仪器提供部分支持。它将被安置在俄亥俄州立大学的P.I.S实验室，在那里它将成为P.I.S地球微生物学、生物地球化学和纳米科学研究项目的主要工具。该仪器将能够收集与光学观测同时进行的力测量以及荧光共振能量转移。此外，它将允许同时收集原子力显微镜(或扫描近场光学显微镜)和激光扫描显微镜图像。例如，该仪器的应用包括：研究活细胞和材料表面(或其他细胞)上的生物分子之间的基本分子内力和分子间作用力；识别与特定矿物或细胞特定相互作用的细胞壁生物分子；研究生物分子在微生物表面的性质和定位；研究矿物的溶解和生长，包括生物矿化；调查金属(或有机酸)在矿物表面的吸附；开发纳米光刻技术；以及教育宣传。

0132433LowerThis grant provides partial support for a state-of-the-art instrument that integrates optical micros-copy, confocal laser scanning microscopy, scanning probe microscopy, and optical tweezers. It will be housed in the P.I.'s laboratory at the Ohio State University, where it will be a primary tool for the P.I.'s research programs in geomicrobiology, biogeochemistry, and nanoscale sci-ence. This instrument will be capable of collecting force measurements concurrent with optical observations as well as fluorescence resonance energy transfer. Further, it will allow the simultaneous collection of atomic force microscopy (or scanning near-field optical microscopy) and laser scanning microscopy images. The applications of this instrument include for example: the study of fundamental intra- and inter-molecular forces between biomolecules on living cells and material surfaces (or other cells); identification of cell wall biomolecules which interact specifically with particular minerals or cells; the study of the properties and localization of biomolecules on microbial surfaces; the study of mineral dissolution and growth including biomineralization; investigations of metal (or organic acid) sorption to mineral surfaces; the development of nanolithography techniques; and educational outreach.***