Acquisition of an integrated scanning probe, scanning laser, optical microscope

这项拨款为集成光学显微复制、共聚焦激光扫描显微镜、扫描探针显微镜和光学镊子的最先进仪器提供部分支持。它将被安置在私家侦探在俄亥俄州立大学（Ohio State University），它将成为私家侦探的主要工具他的研究项目包括地球微生物学、生物地球化学和纳米科学。该仪器将能够收集与光学观测以及荧光共振能量转移同时进行的力测量。此外，它将允许同时收集原子力显微镜（或扫描近场光学显微镜）和激光扫描显微镜图像。该仪器的应用包括：活细胞和材料表面（或其他细胞）上的生物分子之间的基本分子内和分子间力的研究；鉴定与特定矿物质或细胞特异性相互作用的细胞壁生物分子；微生物表面生物分子的性质及其定位研究矿物溶解和生长的研究，包括生物矿化；金属（或有机酸）在矿物表面吸附的研究；纳米光刻技术的发展；还有教育推广

0132433LowerThis grant provides partial support for a state-of-the-art instrument that integrates optical micros-copy, confocal laser scanning microscopy, scanning probe microscopy, and optical tweezers. It will be housed in the P.I.'s laboratory at the Ohio State University, where it will be a primary tool for the P.I.'s research programs in geomicrobiology, biogeochemistry, and nanoscale sci-ence. This instrument will be capable of collecting force measurements concurrent with optical observations as well as fluorescence resonance energy transfer. Further, it will allow the simultaneous collection of atomic force microscopy (or scanning near-field optical microscopy) and laser scanning microscopy images. The applications of this instrument include for example: the study of fundamental intra- and inter-molecular forces between biomolecules on living cells and material surfaces (or other cells); identification of cell wall biomolecules which interact specifically with particular minerals or cells; the study of the properties and localization of biomolecules on microbial surfaces; the study of mineral dissolution and growth including biomineralization; investigations of metal (or organic acid) sorption to mineral surfaces; the development of nanolithography techniques; and educational outreach.***