Electronic Detection of BioMolecules

生物分子的电子检测

基本信息

  • 批准号:
    0415130
  • 负责人:
  • 金额:
    $ 39万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2004
  • 资助国家:
    美国
  • 起止时间:
    2004-08-15 至 2007-07-31
  • 项目状态:
    已结题

项目摘要

This project focuses on the investigation of conformational dynamics of biomolecules using nanoscale electronic structures where the sensing element is a carbon nanotube or a nanotube network. Such sensors are small and fast (the active area is compatible with individual proteins or DNA) and are extremely sensitive since all the current flows at the surface of the nanotube conducting channel, and thus passes through the detection point. The objective of the research is to use these nanoscale electronic devices as bio-sensors and also explore their potential in real-time detection of conformational changes of biomolecules in a buffer environment. Three areas will be explored. First, duplex formation as well as the melting of the DNA duplex will be examined using oligomers of different lengths and sequences. Second, interactions between proteins will be studied using the biotin-streptavidin, ligand-receptor model system. The feasibility of detecting conformational changes will be explored using bacteriorhodopsin as a model system. Third, direct detection of protein binding to nanotubes, and the subsequent conformational changes, will be monitored in real time using devices where individual nanotubes span the source and drain of the electronic device. Experiments on electronic detection will be conducted hand-in-hand with spectroscopic measurements and with direct imaging of the devices. In the broader sense, electronic detection schemes may offer alternatives of optical detection in diverse areas of biotechnology, including gene chips and protein chips, and also medical diagnostics. The electronic detection schemes to be explored may complement other single molecule detection techniques, including optical methods, ESR and NMR techniques.
本项目的重点是利用纳米级电子结构研究生物分子的构象动力学,其中传感元件是碳纳米管或纳米管网络。这种传感器体积小,速度快(活性区域与单个蛋白质或DNA相容),并且非常灵敏,因为所有电流都在纳米管导电通道的表面流动,因此通过检测点。该研究的目的是使用这些纳米电子器件作为生物传感器,并探索其在缓冲环境中实时检测生物分子构象变化的潜力。将探讨三个领域。首先,将使用不同长度和序列的寡聚体检查双链体形成以及DNA双链体的解链。其次,蛋白质之间的相互作用将使用生物素-链霉亲和素,配体-受体模型系统进行研究。以细菌视紫红质为模型系统,探讨其检测构象变化的可行性。第三,蛋白质与纳米管结合的直接检测以及随后的构象变化将使用其中单个纳米管跨越电子器件的源极和漏极的器件来真实的监测。电子探测实验将与光谱测量和设备的直接成像一起进行。在更广泛的意义上,电子检测方案可以在生物技术的不同领域提供光学检测的替代方案,包括基因芯片和蛋白质芯片,以及医疗诊断。待探索的电子检测方案可以补充其他单分子检测技术,包括光学方法、ESR和NMR技术。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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George Gruner其他文献

Dispositifs electroniques actifs a composants composites nanofils
电子装置激活复合材料纳米纤维
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    George Gruner
  • 通讯作者:
    George Gruner

George Gruner的其他文献

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{{ truncateString('George Gruner', 18)}}的其他基金

Optical Spectroscopy of Electronic Matter Close to Quantum Criticality
接近量子临界点的电子物质的光谱学
  • 批准号:
    0454540
  • 财政年份:
    2005
  • 资助金额:
    $ 39万
  • 项目类别:
    Continuing Grant
NER: Fabrication of Electronic Devices with Nanotube Network Conducting Channels
NER:利用纳米管网络导电通道制造电子器件
  • 批准号:
    0404029
  • 财政年份:
    2004
  • 资助金额:
    $ 39万
  • 项目类别:
    Standard Grant
Electrodynamics of Electron Liquids and Electron Glasses
电子液体和电子玻璃的电动力学
  • 批准号:
    0102405
  • 财政年份:
    2001
  • 资助金额:
    $ 39万
  • 项目类别:
    Continuing Grant
Investigation of Charge Transport Along the DNA Double Helix
沿 DNA 双螺旋的电荷传输研究
  • 批准号:
    0077251
  • 财政年份:
    2000
  • 资助金额:
    $ 39万
  • 项目类别:
    Standard Grant
Low Frequency Electrodynamics of Correlated Metals
相关金属的低频电动力学
  • 批准号:
    9801816
  • 财政年份:
    1998
  • 资助金额:
    $ 39万
  • 项目类别:
    Continuing Grant
Experiments on the Electrodynamics of Charged Quantum Liquids
带电量子液体的电动力学实验
  • 批准号:
    9503009
  • 财政年份:
    1995
  • 资助金额:
    $ 39万
  • 项目类别:
    Continuing Grant
US-Hungary Materials Research on Properties of Low Dimensional Solids
美国-匈牙利低维固体特性材料研究
  • 批准号:
    9322517
  • 财政年份:
    1994
  • 资助金额:
    $ 39万
  • 项目类别:
    Standard Grant
Acquisition of a Low Temperature and High Magnetic Field Optical Facility
购置低温高磁场光学设施
  • 批准号:
    9404319
  • 财政年份:
    1994
  • 资助金额:
    $ 39万
  • 项目类别:
    Standard Grant
Millimeter and Submillimeter Spectroscopy of Solids
固体的毫米波和亚毫米波谱
  • 批准号:
    9216500
  • 财政年份:
    1993
  • 资助金额:
    $ 39万
  • 项目类别:
    Standard Grant
An Experimental Investigation of Charge and Spin Density Waves
电荷和自旋密度波的实验研究
  • 批准号:
    9218745
  • 财政年份:
    1993
  • 资助金额:
    $ 39万
  • 项目类别:
    Continuing Grant

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