Kinetic and Thermodynamic Studies Relevant to Biological Iron Transport

与生物铁运输相关的动力学和热力学研究

• 批准号: 0418006
• 负责人: Alvin Crumbliss
• 金额: $ 45.4万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-15 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0418006&HistoricalAwards=false
• 关键词: Kinetic; Thermodynamic; Studies; Relevant; Biological

Professor A. L. Crumbliss of Duke University will investigate biological iron transport mechanisms. The research plan will employ in vitro methods to probe the mechanism of iron transport mediated by bacterial Fbp and mammalian Tf proteins. Natural processes have evolved to efficiently sequester, transport and store iron, and studies of iron speciation, coordination, and ligand exchange will contribute to unraveling the details of biological iron manipulation. Multiple techniques will be employed to monitor the dynamics of iron transport, and MALDI-TOF SUPREX will be used to determine binding affinities. The working hypothesis that reduction of Fe(III) promotes release of iron from transferrin will be tested.Natural processes have developed to control the uptake, transport and storage of iron. Iron manipulation is essential in many biological sytems. This project will probe fundamental inorganic chemical reactions that regulate iron mobility in selected proteins and small molecules.

A教授L.杜克大学的Crumbliss将研究生物铁转运机制。 该研究计划将采用体外方法来探索细菌Fbp和哺乳动物Tf蛋白介导的铁转运机制。自然过程已经发展到有效地螯合，运输和储存铁，铁形态，协调和配体交换的研究将有助于解开生物铁操纵的细节。将采用多种技术监测铁转运的动力学，并将使用MALDI-TOF SUPREX测定结合亲和力。 铁（III）的还原促进铁从转铁蛋白中释放的工作假设将被测试。自然过程已经发展到控制铁的吸收，运输和储存。 铁操纵在许多生物系统中是必不可少的。 该项目将探索调节选定蛋白质和小分子中铁流动性的基本无机化学反应。