Kinetic and Thermodynamic Studies Relevant to Biological Iron Transport

杜克大学的A.L.Crumbliss教授将研究生物铁的运输机制。该研究计划将使用体外方法来探索细菌FBP和哺乳动物TF蛋白介导的铁转运机制。自然过程已经进化为有效地隔离、运输和储存铁，铁的形态、配位和配体交换的研究将有助于揭开生物铁操纵的细节。将使用多种技术来监测铁的运输动力学，并将使用MALDI-TOF SUPREX来确定结合亲和力。铁(III)还原促进铁从转铁蛋白中释放的工作假说将得到验证。已发展出控制铁的吸收、运输和储存的自然过程。铁的操控在许多生物系统中是必不可少的。该项目将探索调节选定蛋白质和小分子中铁流动性的基本无机化学反应。

Professor A. L. Crumbliss of Duke University will investigate biological iron transport mechanisms. The research plan will employ in vitro methods to probe the mechanism of iron transport mediated by bacterial Fbp and mammalian Tf proteins. Natural processes have evolved to efficiently sequester, transport and store iron, and studies of iron speciation, coordination, and ligand exchange will contribute to unraveling the details of biological iron manipulation. Multiple techniques will be employed to monitor the dynamics of iron transport, and MALDI-TOF SUPREX will be used to determine binding affinities. The working hypothesis that reduction of Fe(III) promotes release of iron from transferrin will be tested.Natural processes have developed to control the uptake, transport and storage of iron. Iron manipulation is essential in many biological sytems. This project will probe fundamental inorganic chemical reactions that regulate iron mobility in selected proteins and small molecules.