Aradbidopsis 2010: Tools and Technologies to Enable Genome-Wide Screens in Arabidopsis

本研究涉及植物生物学研究工具和资源的开发。对拟南芥基因组序列的分析揭示了大约25,000个蛋白质编码基因的存在。现在应该有可能进行系统的全基因组功能筛选，以评估每个基因对生物过程的贡献。然而，由于缺乏一套完整的基因索引纯合突变体，无偏倚的全基因组功能筛选尚不可行。为了满足这些需求，这个2010年项目的目标是通过实验鉴定25000个拟南芥基因的两个纯合插入突变体，从而创建一个“表型就绪”的基因组资源。无偏倚的全基因组筛选将使基因组注释具有关于任何基因的调控和生物学功能的新的高质量信息。该项目开发的资源将对所有研究人员开放，并将为依赖全基因组信息的各种项目提供基础。该项目的数据可在http://signal.salk.edu上找到，种子将提供给拟南芥生物资源中心（http://www.biosci.ohio-state.edu/~plantbio/Facilities/abrc/abrchome.htm）进行分发。该项目的另一个重要特点是对本科生进行生物信息学和基因组学方法的培训。这些突变体对农业的长期影响是能够培育出具有优良农艺性状的植物。

The proposed study pertains to the development of research tools and resources for the plant biology community. Analysis of the genome sequence of Arabidopsis thaliana revealed the existence of approximately 25,000 protein-coding genes. It should now be possible to undertake systematic genome-wide functional screens that assess the contribution of every gene to a biological process. However, due to the absence of a complete set of gene-indexed homozygous mutants unbiased genome-wide functional screens are not yet feasible. To fulfill these needs, the aim of this 2010 project is to experimentally identity two homozygous insertion mutants for 25,000 Arabidopsis genes, thereby creating a "phenome-ready" genomics resource. Unbiased genome-wide screens will enable annotation of the genome with new high-quality information about the regulation and biological functions of any gene. The resources developed by this project will be available to all researchers and will provide the basis for a variety of projects that rely upon whole genome information. The data from this project can be found at: http://signal.salk.edu, and seed will be provided to the Arabidopsis Biological Resource Center (http://www.biosci.ohio-state.edu/~plantbio/Facilities/abrc/abrchome.htm) for distribution. Another important feature of the project is the training of undergraduate students in bioinformatics and genomic methodologies. The long-term impact of these mutants on agriculture is to enable construction of plants with superior agronomic traits.