Aquisition of a Protein Fractionation System for Research and Education

获取用于研究和教育的蛋白质分离系统

• 批准号: 0420581
• 负责人: Phillip Danielson
• 金额: --
• 依托单位: University of Denver
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0420581&HistoricalAwards=false
• 关键词: Aquisition; Protein; Fractionation; System; Research

A grant has been awarded to the University of Denver under the direction of Dr. Phillip Danielson for partial support of the purchase of a Protein Fractionation System. The accurate fractionation, quantization, recovery and characterization of individual proteins from complex proteomes are capabilities that are increasingly essential to the growth and success of biological research and education. Until recently, the analysis of whole proteomes has been heavily dependent on 2-Dimensional Gel Electrophoresis (2DGE)-based approaches. This approach required the laborious screening of hundreds to thousands of resolved "spots" on thin gels. The identification of even a small number of proteins of interest, can require weeks to months to complete. These more traditional methods have several critical shortcomings. 2DGE provides poor resolution of the small peptide hormones and larger membrane-associated proteins that are the focus of many of researchers programs. Furthermore, 2DGE yields results that are often difficult to quantify or reproduce. The Protein Fractionation System provides a cost-effective solution to the traditional limitations of 2DGE-based proteomic research. Test data from difficult samples have confirmed the performance and applicability of the system for our research needs. Specific research programs that will benefit immediately include NSF-funded studies of the posttranslational processing and modification of neuropeptide hormones involved in the management of reproductive stress and proteomic research aimed at elucidating the underlying neuroendocrine mechanisms of mammalian feeding behavior. Other studies are examining the molecular neurobiology of mammalian taste cells, the recycling of neuroendocrine secretory vesicles and the degradation of misfolded proteins. Finally the Protein Fractionation system will advance collaborative research in molecular ecology and conservation biology conducted in collaboration with the Denver Botanical Gardens. Beyond the benefit to basic research at the University of Denver, a broad range of laboratory and classroom-oriented educational goals will be advanced at both the undergraduate and graduate levels. Benefits will be particularly evident in the molecular-oriented laboratory courses that are at the heart of the Bachelor of Science and Bachelor of Arts degrees in Molecular Biology. On a broader level, department-sponsored biotechnology classes offered to high school students and teacher-training workshops that promote hands-on science education at the secondary school level will also be greatly enhanced by providing first-hand experience in one of the most modern methods of proteomic analysis. The benefit to high school outreach efforts will be immeasurable given that these programs target students in urban and low-income school districts who have traditionally been underrepresented in the natural sciences. Most importantly, exposing pre-college students to modern technologies will have a significant and positive impact on student excitement about science as a career.

在菲利普丹尼尔森博士的指导下，丹佛大学获得了一笔赠款，用于部分支持购买蛋白质分馏系统。从复杂蛋白质组中精确分离、量化、回收和表征单个蛋白质是生物研究和教育的增长和成功越来越重要的能力。直到最近，整个蛋白质组的分析一直严重依赖于二维凝胶电泳（2DGE）为基础的方法。这种方法需要在薄凝胶上费力地筛选数百到数千个分辨的“斑点”。即使是少量感兴趣的蛋白质的鉴定也可能需要数周至数月才能完成。 这些更传统的方法有几个关键的缺点。2DGE对小肽激素和较大的膜相关蛋白的分辨率较差，这些蛋白是许多研究人员计划的重点。此外，2DGE产生的结果往往难以量化或再现。蛋白质分级系统为基于2DGE的蛋白质组学研究的传统局限性提供了一种具有成本效益的解决方案。从困难的样品的测试数据已经证实了我们的研究需要的系统的性能和适用性。将立即受益的具体研究计划包括NSF资助的神经肽激素翻译后加工和修饰的研究，这些研究涉及生殖应激的管理和蛋白质组学研究，旨在阐明哺乳动物摄食行为的潜在神经内分泌机制。其他研究正在研究哺乳动物味觉细胞的分子神经生物学，神经内分泌囊泡的再循环和错误折叠蛋白质的降解。最后，蛋白质分级系统将推进与丹佛植物园合作进行的分子生态学和保护生物学的合作研究。除了有利于丹佛大学的基础研究之外，还将在本科和研究生阶段推进广泛的实验室和课堂教育目标。好处将是特别明显的分子为导向的实验室课程，是在科学和文学学士学位的分子生物学学士学位的核心。 在更广泛的层面上，部门主办的生物技术课程提供给高中学生和教师培训讲习班，促进在中学一级的动手科学教育也将大大加强提供第一手的经验，在蛋白质组学分析的最现代的方法之一。考虑到这些项目针对的是城市和低收入学区的学生，这些学生传统上在自然科学方面的代表性不足，因此高中外展工作的好处将是不可估量的。最重要的是，让大学预科学生接触现代技术将对学生对科学作为职业的兴奋产生重大而积极的影响。