Acquisition of an Automated DNA Sequencing and Sample Prep Core Unit for Research and Education

收购用于研究和教育的自动 DNA 测序和样品制备核心单元

• 批准号: 9977691
• 负责人: Phillip Danielson
• 金额: $ 12.68万
• 依托单位: University of Denver
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-15 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9977691&HistoricalAwards=false
• 关键词: Acquisition; Automated; DNA; Sequencing; Sample

AWARD ABSTRACT - 9977691 This award will fund the purchase of a Beckman Coulter CEQ 2000Automated DNA Sequencing and Sample Prep Core Unit. Based onwell-established DNA sequencing technology, this system will provide for asignificant increase in the productivity and cost effectiveness ofundergraduate/graduate research and education in molecular biology at theUniversity of Denver. Specific research programs that will benefitimmediately include National Science Foundation-funded studies on: themolecular evolution of endocrine neuropeptide hormones, avian sexchromosomes, and insect steroidogenesis; the population genetics ofendangered species; the use of site-directed mutagenesis to delineate thestructural properties of proteins; and the regulation and ecologicalsignificance of the cytochrome P450 superfamily of toxin-metabolizingenzymes. Additionally, use of the automated sequencer in collaborativework in conservation genetics with researchers from the Denver zoologicalgardens, will further studies of the genetic diversity of endangered andthreatened species. Beyond the in-depth training of undergraduate and graduatestudents in the research laboratory setting, a broad range ofclassroom-oriented educational goals will be advanced. Benefits will beparticularly evident in the molecular-oriented laboratory courses that areat the heart of the new Bachelor of Science and Bachelor of Arts degreesin Molecular Biology offered by the Department of Biological Sciences.Department-sponsored biotechnology classes offered to high school studentsand teacher-training workshops that promote hands-on science education atthe secondary school level will also be greatly enhanced. The benefit tohigh school outreach efforts is immeasurable given that these programstarget students in urban and low-income school districts who havetraditionally been underrepresented in the natural sciences. In short, acquisition of the CEQ 2000 DNA Sequencing and SamplePrep Core Unit will provide significant and immediate benefits toeducation at the secondary, undergraduate and graduate levels, whileproviding a cost-efficient means of satisfying the growing DNA sequencingneeds of researchers funded by the National Science Foundation in both theDepartment of Biological Sciences and the Department of Chemistry andBiochemistry.

奖项摘要- 9977691该奖项将资助购买贝克曼库尔特CEQ 2000自动DNA测序和样品制备核心单元。基于成熟的DNA测序技术，该系统将为丹佛大学的研究生/研究生分子生物学研究和教育的生产力和成本效益提供显着提高。具体的研究计划，将受益包括国家科学基金会资助的研究：内分泌神经肽激素，鸟类性染色体和昆虫类固醇的分子进化;濒危物种的群体遗传学;使用定点突变来描绘蛋白质的结构特性;和调节和生态意义的细胞色素P450超家族的毒素代谢酶。此外，在与丹佛动物园的研究人员的保护遗传学合作中使用自动测序仪，将进一步研究濒危和受威胁物种的遗传多样性。 除了在研究实验室环境中对本科生和研究生进行深入培训外，还将推进广泛的课堂教育目标。生物科学系提供的分子生物学理学士和文学士学位的核心--以分子为导向的实验室课程的好处将尤为明显。生物科学系为高中生提供的生物技术课程和促进中学科学实践教育的教师培训讲习班也将大大加强。考虑到这些计划的目标是城市和低收入学区的学生，这些学生传统上在自然科学方面的代表性不足，因此高中推广工作的好处是不可估量的。简而言之，收购CEQ 2000 DNA测序和样品制备核心单元将为中学、本科和研究生教育提供显著和直接的好处，同时提供一种符合成本效益的方法，满足生物科学系和化学与生物化学系由国家科学基金会资助的研究人员日益增长的DNA测序需求。