Fractionation of sweet lupin protein and study of physicochemical and functional properties of obtained isolates in food systems

甜罗苹种子富含蛋白质，具有良好的功能和营养特性，多年来引起了食品工业的兴趣。然而，到目前为止，由于缺乏对蛋白质的全面表征和功能筛选，这些蛋白质尚未在食品部门充分发挥其潜力。鲍威尔梅国际（PMI）是一家总部位于加拿大的原料经纪人，希望在其产品组合中添加甜罗苹种子蛋白。因此，本项目的目的是开发出一种食品级的分离方法来分离甜罗苹种子蛋白，并对分离蛋白进行全面的表征。表征包括对所获得的分离物进行全面的物理化学和功能研究。该项目是将一种被低估的蛋白质来源商业化的第一步，这种蛋白质来源可以在安大略省种植。该项目对加拿大的及时性和重要性可以通过以下方面的潜力来说明：(i)将加拿大的PMI置于国际植物性蛋白质产业的前沿；（ii）对一种作物的更高估值，可以被加拿大/安大略省的农民纳入作物轮作计划。此外，该项目恰逢行业和消费者对具有优良营养特性的替代植物性蛋白质的更大需求。

Sweet lupin seeds as rich source of protein with promising functional and nutritional properties have triggeredthe interest of the food industry for a number of years now. However, to date, these proteins have not yet beenexploited to their full potential in the food sector as a thorough characterization and functionality screening ofthe protein is lacking. Powell May International (PMI) is a Canada-based ingredient broker which would like toadd sweet lupin seed protein to its product portfolio. The objective of this project is, hence, to develop afood-grade isolation procedure for the sweet lupin seed proteins and a thorough characterization of the proteinisolate. The characterization involves a full physicochemical and functional study of the obtained isolates. Thisproject is a first step towards possible commercialization of an undervalued protein source that can be grown inOntario. The timeliness and importance of this project for Canada can be illustrated by the potential this projecthas in terms of (i) putting Canada-based PMI at the forefront of the international plant-based protein industryand (ii) higher valuation of a crop that can be incorporated in a crop rotation plan by farmers inCanada/Ontario. Furthermore, this project coincides with the greater demand of both industry as well asconsumers for alternative plant-based proteins with excellent nutritional properties.