Fractionation of sweet lupin protein and study of physicochemical and functional properties of obtained isolates in food systems

甜羽扇豆子作为丰富的蛋白质来源，具有良好的功能和营养特性，多年来一直引起食品工业的兴趣。然而，到目前为止，这些蛋白质还没有在食品领域得到充分的开发，因为缺乏对蛋白质的彻底表征和功能筛选。鲍威尔·梅国际公司(PMI)是一家总部位于加拿大的配料经纪商，希望将甜羽扇豆籽蛋白添加到其产品组合中。因此，本项目的目标是开发一种食品级的甜羽扇豆籽蛋白的分离程序，并对该分离蛋白进行彻底的表征。鉴定包括对获得的分离株进行全面的物理化学和功能研究。这个项目是朝着可能的商业化迈出的第一步，这种被低估的蛋白质资源可以在安大略省种植。这个项目对加拿大的及时性和重要性可以从以下方面体现出来：(I)使加拿大的PMI走在国际植物蛋白工业的前列，(Ii)加拿大/安大略省农民可以将作物纳入轮作计划的更高估值。此外，该项目与工业和消费者对具有良好营养特性的替代植物蛋白的更大需求不谋而合。

Sweet lupin seeds as rich source of protein with promising functional and nutritional properties have triggeredthe interest of the food industry for a number of years now. However, to date, these proteins have not yet beenexploited to their full potential in the food sector as a thorough characterization and functionality screening ofthe protein is lacking. Powell May International (PMI) is a Canada-based ingredient broker which would like toadd sweet lupin seed protein to its product portfolio. The objective of this project is, hence, to develop afood-grade isolation procedure for the sweet lupin seed proteins and a thorough characterization of the proteinisolate. The characterization involves a full physicochemical and functional study of the obtained isolates. Thisproject is a first step towards possible commercialization of an undervalued protein source that can be grown inOntario. The timeliness and importance of this project for Canada can be illustrated by the potential this projecthas in terms of (i) putting Canada-based PMI at the forefront of the international plant-based protein industryand (ii) higher valuation of a crop that can be incorporated in a crop rotation plan by farmers inCanada/Ontario. Furthermore, this project coincides with the greater demand of both industry as well asconsumers for alternative plant-based proteins with excellent nutritional properties.