Acquisition of DNA Sequencer

在这份提案中，我们要求为Perkin-Elmer/应用生物系统公司373A-01型自动测序仪提供资金。这台机器将主要由四个实验室组成的小组使用，也将向植物生物学系和环境科学、政策和管理部的其他用户开放。以前使用借出的机器的经验表明，我们将有效地使用它。空间、支持人员和集体资源可用于永久住房和机器维护。伯克利校区目前没有核心测序设施。这台机器将使校园中的永久机器数量翻一番，也将是两个部门成员唯一可用的机器。使用借来的自动测序仪加快了我们目前由NSF赞助的工作的速度，也促进了微生物生态学、种群遗传学、植物发育和植物抗病领域新方法的创新或先前预期的扩展。很明显，自动测序很快就会成为像我们这样的实验室的首选方法，在我们的实验室中，序列获取是一个速度限制步骤，因此它也是研究生和博士后助理应该学习的方法。

In this proposal we request funding for a Perkin-Elmer / Applied Biosystems Automated Sequencer model 373A-01 . This machine will be used primarily by a group of four labs and will be also open to other users in the Department of Plant Biology and the Department of Environmental Science, Policy, and Management. Prior experience with a loaned machine demonstrates that we will make efficient use of it. Space, support staff, and collective resources are available for permanent housing and maintenance of the machine. There is currently no core sequencing facility on the Berkeley campus. This machine would double the number of permanent machines on campus and would be the only one available to members of either department . Access to borrowed automated sequencer has accelerated the rate at which we conduct our current NSF sponsored work and has also facilitated innovations of new approaches or expansions of prior expectations in the fields of microbial ecology, population genetics, plant development, and plant disease resistance. It is clear that automated sequencing will soon be the method of choice for labs such as ours in which sequence acquisition is a rate limiting step, and as such it is also the method that graduate students and postdoctoral associates should learn.