Acquisition of an Automated DNA Sequencer for Advanced Biomolecular Research

采购用于高级生物分子研究的自动 DNA 测序仪

• 批准号: 9870959
• 负责人: Gabor Mocz
• 金额: $ 10万
• 依托单位: University of Hawaii
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-10-01 至 2000-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9870959&HistoricalAwards=false
• 关键词: Acquisition; Automated; DNA; Sequencer; Advanced

9870959Gabor Mocz University of Hawaii ManoaAcquisition of an Automated DNA Sequencer for Advanced Biomolecular ResearchDNA sequence analysis has become an essential tool in many areas of biological research. This award funds the purchase of a Perkin-Elmer ABI 377 DNA sequencer to be used by researchers in biology at the University of Hawaii. The sequencer will be placed in the Biotechnology/Molecular Biology Instrumentation and Training facility. The projects to be supported by this instrument include (1) exotic pest identification using genetic methods based on the polymerase chain reaction, (2) identification of symbiosis-induced bacterial genes in Vibrio fischeri, (3) molecular systematics of Hawaiian flora, (4) plant transformation technology for reduced variation of transgene expression, and (5) site directed mutagenesis and protein expression system to produce novel human serum albumin species. These projects, and others making use of the new DNA sequencer, will enhance not only the research infrastructure at the University of Hawaii, but also training efforts through the Pacific Biomedical Research Center and the Research Centers in Minority Institutions Program.

夏威夷大学马诺获得用于高级生物分子研究的自动DNA测序仪DNA序列分析已成为许多生物研究领域的重要工具。 该奖项资助购买珀金埃尔默ABI 377 DNA测序仪，供夏威夷大学生物学研究人员使用。 测序仪将放置在生物技术/分子生物学仪器和培训机构。 该工具支持的项目包括：（1）利用基于聚合酶链反应的遗传方法鉴定外来害虫，（2）鉴定费氏弧菌中共生诱导的细菌基因，（3）夏威夷植物群的分子系统学，（4）减少转基因表达变异的植物转化技术，和（5）定点突变和蛋白表达系统以产生新的人血清白蛋白种类。 这些项目和其他利用新的DNA测序仪的项目不仅将加强夏威夷大学的研究基础设施，而且还将加强太平洋生物医学研究中心和少数民族机构研究中心方案的培训工作。