Acquisition of a Raman Microscope

购买拉曼显微镜

• 批准号: 0420739
• 负责人: Timothy Elgren
• 金额: $ 21.15万
• 依托单位: Hamilton College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0420739&HistoricalAwards=false
• 关键词: Acquisition; Raman; Microscope

With support from the Major Research Instrumentation (MRI) Program, the Department of Chemistry at Hamilton College will acquire a dispersive Raman spectrometer equipped with a confocal Raman microscope and a macro sampling chamber. This equipment will enhance research in a number of areas: a) Raman probe of enzyme mechanism and active site structure; b) studies of biological transformations of iron oxide minerals by dissimilative iron reducing bacteria; and c) maintaining biofunctionality of microcontact printed protein nanostructures. Raman spectroscopy provides a powerful probe of molecular information. Raman scattering is complementary to infrared absorption in that it probes fundamentally different types of molecular vibrational modes. For biological samples, Raman scattering is the preferred method because it is not affected by the polar water environment that absorbs strongly in the infrared. The Raman effect is enhanced when coupled to a charge-transfer transition or when samples are adsorbed on a metal surface. These enhancement mechanisms can lead to a million-fold increase in signal intensity. This instrument will be used not only for research but also for a number of courses at this undergraduate institution.

在主要研究仪器（MRI）计划的支持下，汉密尔顿学院的化学系将获得一台色散拉曼光谱仪，该光谱仪配备有共焦拉曼显微镜和宏观取样室。该设备将加强若干领域的研究：a）酶机制和活性部位结构的拉曼探针; B）异化铁还原菌对氧化铁矿物的生物转化研究; c）保持微接触打印蛋白质纳米结构的生物功能。拉曼光谱学提供了一种强有力的分子信息探针。 拉曼散射与红外吸收互补，因为它探测根本不同类型的分子振动模式。 对于生物样品，拉曼散射是优选的方法，因为它不受在红外中强烈吸收的极性水环境的影响。 拉曼效应增强时，耦合到一个电荷转移过渡或当样品吸附在金属表面上。 这些增强机制可以导致信号强度增加一百万倍。 该仪器将不仅用于研究，而且还用于该本科院校的一些课程。