Acquisition of a confocal Raman microscope for molecular fingerprinting of cells and tissue

获取用于细胞和组织分子指纹分析的共焦拉曼显微镜

• 批准号: 10582119
• 负责人: Kyle Patrick Quinn
• 金额: $ 20.05万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10582119
• 关键词: Arkansas; Bioenergetics; Biological; Biomedical Research; Cells; Centers of Research Excellence; Complex; Data Science; Development; Disease; Fingerprint; Future; Goals; Heterogeneity; Image; Interdisciplinary Study; Label; Metabolic; Metabolism; Microscope; Microscopy; Molecular; Molecular Profiling; Organism; Oxidation-Reduction; Oxygen; Phase; Pilot Projects; Research; Research Project Grants; Role; Specimen; Spectrum Analysis; Techniques; Tissues; Universities; cellular imaging; light scattering; metabolic imaging; molecular imaging; repaired; spectroscopic imaging; two photon microscopy; vibration

PROJECT SUMMARY The Arkansas Integrative Metabolic Research Center (AIMRC) is a Phase I COBRE at the University of Arkansas at Fayetteville (04/01/2021-02/28/2026; PI: Dr. Kyle P. Quinn). The scientific theme of the AIMRC is to understand the role of cell and tissue metabolism in disease, development, and repair through research involving advanced imaging, bioenergetics, and data science. The long-term goal of the AIMRC is to establish a sustainable interdisciplinary research center that can support biomedical research at the U of A and grow the emerging strength in metabolic research on campus. The Imaging and Spectroscopy Core will enable label-free non-destructive imaging of cellular redox status and oxygen saturation in live cells, tissues, and organisms through an assortment of microscopy and spectroscopy techniques. In addition to our existing capabilities, we propose to install a confocal Raman microscope for non-destructive and label-free imaging of the molecular composition of cells, tissues, and tissue sections. Raman scattering offers the ability to probe biomolecular changes and visualize the complex molecular heterogeneity directly from cells and tissues. The technique relies on the inelastic scattering of light, arising from its interactions with the biological specimen, to quantify the unique vibrational modes of molecules within its native context. Since every molecular species has a unique Raman scattering spectrum or “fingerprint”, analyses of cells and tissues using the Raman microscope can provide highly complementary information to the quantitative metabolic imaging by two-photon microscopy. The Raman microscope purchased as part of this supplement request will be highly beneficial for the projects proposed by current research project leaders and pilot project awardees who may be candidates for future advancement to research project leaders.

项目总结 阿肯色州综合代谢研究中心(AIMRC)是阿肯色州大学的一期工程 在费耶特维尔(2021年4月1日-2026年2月28日；PI：Kyle P.Quinn博士)。AIMRC的科学主题是 通过研究了解细胞和组织代谢在疾病、发育和修复中的作用 先进的成像、生物能量学和数据科学。AIMRC的长期目标是建立一个 可持续的跨学科研究中心，可以支持加州大学的生物医学研究，并发展 在校园新陈代谢研究方面的新兴力量。成像和光谱核心将实现无标签 活细胞、组织和生物体中细胞氧化还原状态和氧饱和度的非破坏性成像 通过一系列显微镜和光谱学技术。除了我们现有的能力外，我们还 建议安装共聚焦拉曼显微镜，用于分子的非破坏性和无标记成像 细胞、组织和组织切片的组成。拉曼散射提供了探测生物分子的能力 直接从细胞和组织改变和可视化复杂的分子异质性。这项技术依靠 关于光与生物样本相互作用产生的非弹性散射，以量化独特的 分子在其自然环境中的振动模式。因为每个分子物种都有一个独特的拉曼光谱 利用拉曼显微镜对细胞和组织进行的散射光谱或“指纹”分析可以提供高度的 补充双光子显微镜定量代谢成像的信息。拉曼 作为本补充申请的一部分购买的显微镜将对以下项目非常有益 现任研究项目负责人和试点项目获奖者，他们可能是未来晋升的候选人 研究项目负责人。