MRI: Development of An In-situ Flow Cytometer for Enhanced Aquatic Particle Discrimination

MRI：开发用于增强水生颗粒辨别的原位流式细胞仪

• 批准号: 0421310
• 负责人: James Bonner
• 金额: --
• 依托单位: Texas A&M Engineering Experiment Station
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421310&HistoricalAwards=false
• 关键词: MRI; Development; situ; Flow; Cytometer

Proposal No. CTS-0421310Principal Investigator: J. S. Bonner, Texas A&M University, Corpus ChristiThis grant will result in improved in situ flow cytometry instrumentation to enable real-time particle detection (colloidal, super-colloidal and microbial) in coastal margin environments. Coastal margins provide ecological and economic services of vast importance to society at large; advancing methods to ensure their sustainable management must continue to be a research and operational priority. It is widely recognized that real-time monitoring is needed for adequate detection of contaminants in coastal margin ecosystems. Furthermore, because of the complexity of interacting physical processes in these ecosystems, static monitoring systems are unable to resolve spatial variability at scales necessary for proper environmental management. Current flow cytometry technology allows for particle discrimination over time at a fixed location. It does not, however, support real-time detection over broader spatial scales. This is a critical limitation, and one that has hampered progress in protection of marine ecosystems from both natural and anthropogenic disturbances. The goal is to modify the commercial FlowCAM cytometer and its procedures to allow continuous marine monitoring over both space and time. A functional flow cytometer will be developed that can be housed in a tube approximately 9 inches in diameter and 3 feet in length. The instrument will be fitted for use on a towable platform for shallow marine environments. The modifications proposed here will allow: 1) quantitative aquatic particle differentiation; 2) rapid instrument deployment and enhanced mobility for use in shallow water environments; 3) synergy with related coastal monitoring sensors; 4) integration with existing databases; and 5) internet capabilities with data streams to the World Wide Web. The potential for broader impacts includes immediate alignment with an existing CRCD project focused on creating a GIS-trained student able to use various data streams for accurate data synthesis modeling with a final capstone course combining both concepts into a real world situation. Further dissemination will occur through existing channels created by the RESSACA CREST and an existing REU Site. Texas A&M University-Corpus Christi is located in south Texas where the population demographics are heavily Hispanic, underrepresented groups will be provided opportunity to pursue advanced degrees in science and engineering as part of their research training and experience. The project will result in improved flow cytometry instrumentation that advances continuous monitoring of coastal margin environments, supporting research, education, and public policy designed to protect coastal margin ecosystems.

提案编号CTS-0421310 PRINCIPAL研究者：德克萨斯州A＆M大学的J. S. Bonner，Cersus Christithis Grant将导致沿海边缘环境中的实时粒子检测（胶体，超胶体和微生物）的现场流式细胞仪仪器改善。沿海边缘为整个社会提供了广泛的生态和经济服务；确保其可持续管理的推进方法必须继续成为研究和运营优先事项。 广泛认识到，需要实时监测才能充分检测沿海边缘生态系统中的污染物。 此外，由于这些生态系统中相互作用的物理过程的复杂性，静态监测系统无法在适当的环境管理所需的尺度上解决空间可变性。 当前的流式细胞仪技术允许在固定位置随着时间的推移进行粒子歧视。 但是，它不支持更广泛的空间尺度上的实时检测。这是一个关键的局限性，它阻碍了保护海洋生态系统免受天然和人为障碍的保护进展。 目的是修改商业流动柜细胞仪及其程序，以允许在空间和时间上进行连续的海洋监测。 将开发一个功能流式细胞仪，该流式细胞仪可容纳在直径约9英寸的管中，长度为3英尺。 该仪器将安装在浅海环境中的可拖动平台上。这里提出的修改将允许：1）定量水生颗粒分化； 2）在浅水环境中使用快速仪器的部署和增强的流动性； 3）与相关的沿海监测传感器协同作用； 4）与现有数据库集成； 5）具有数据流到万维网的互联网功能。 更广泛的影响的潜力包括与现有的CRCD项目的立即保持一致，该项目致力于创建一个由GIS培训的学生，能够使用各种数据流进行准确的数据综合建模，并结合了将这两个概念结合到现实世界状况的最终帽子课程。 将通过Ressaca Crest和现有REU站点创建的现有渠道进行进一步的传播。 德克萨斯州农工大学 - 科普斯·克里斯蒂（Christi）位于德克萨斯州南部，在那里，人口统计资料是西班牙裔大量，代表性不足的团体将有机会在其研究培训和经验的一部分中攻读科学和工程学的高级学位。 该项目将改善流式细胞仪仪器，从而持续监测沿海边缘环境，支持旨在保护沿海边缘生态系统的研究，教育和公共政策。