MRI: Development of An In-situ Flow Cytometer for Enhanced Aquatic Particle Discrimination

建议没有。主要研究人员：J. S. Bonner, Texas a&m University, Corpus christi .这项拨款将改进原位流式细胞仪，使沿海边缘环境中的实时颗粒检测（胶体、超胶体和微生物）成为可能。沿海边缘提供对整个社会极为重要的生态和经济服务；推进确保可持续管理的方法必须继续成为研究和业务的优先事项。人们普遍认识到，为了充分探测沿海边缘生态系统中的污染物，需要进行实时监测。此外，由于这些生态系统中相互作用的物理过程的复杂性，静态监测系统无法在适当的环境管理所需的尺度上解决空间变异性。当前的流式细胞术技术允许在固定位置随着时间的推移进行颗粒识别。然而，它不支持在更大的空间尺度上的实时检测。这是一个严重的限制，阻碍了在保护海洋生态系统免受自然和人为干扰方面取得进展。目标是改进商用流式细胞仪及其程序，以便在空间和时间上进行连续的海洋监测。一种功能流式细胞仪将被开发，可以安置在一个直径约9英寸，长度约3英尺的管。该仪器将安装在浅海环境的可拖曳平台上使用。本文提出的修改将允许：1)定量的水生颗粒分化；2)在浅水环境中快速部署仪器和增强机动性；3)与相关海岸监测传感器协同；4)与现有数据库的集成；5)数据流到万维网的互联网功能。潜在的更广泛影响包括立即与现有的CRCD项目保持一致，该项目的重点是培养能够使用各种数据流进行准确数据合成建模的地理信息系统培训学生，最终的顶点课程将这两个概念结合到现实世界中。进一步的传播将通过RESSACA CREST和现有的REU网站建立的现有渠道进行。德克萨斯农工大学科珀斯克里斯蒂分校位于德克萨斯州南部，那里的人口主要是西班牙裔，因此，作为他们研究培训和经验的一部分，该大学将为未被充分代表的群体提供攻读科学和工程高级学位的机会。该项目将改进流式细胞仪仪器，推进沿海边缘环境的持续监测，支持旨在保护沿海边缘生态系统的研究、教育和公共政策。

Proposal No. CTS-0421310Principal Investigator: J. S. Bonner, Texas A&M University, Corpus ChristiThis grant will result in improved in situ flow cytometry instrumentation to enable real-time particle detection (colloidal, super-colloidal and microbial) in coastal margin environments. Coastal margins provide ecological and economic services of vast importance to society at large; advancing methods to ensure their sustainable management must continue to be a research and operational priority. It is widely recognized that real-time monitoring is needed for adequate detection of contaminants in coastal margin ecosystems. Furthermore, because of the complexity of interacting physical processes in these ecosystems, static monitoring systems are unable to resolve spatial variability at scales necessary for proper environmental management. Current flow cytometry technology allows for particle discrimination over time at a fixed location. It does not, however, support real-time detection over broader spatial scales. This is a critical limitation, and one that has hampered progress in protection of marine ecosystems from both natural and anthropogenic disturbances. The goal is to modify the commercial FlowCAM cytometer and its procedures to allow continuous marine monitoring over both space and time. A functional flow cytometer will be developed that can be housed in a tube approximately 9 inches in diameter and 3 feet in length. The instrument will be fitted for use on a towable platform for shallow marine environments. The modifications proposed here will allow: 1) quantitative aquatic particle differentiation; 2) rapid instrument deployment and enhanced mobility for use in shallow water environments; 3) synergy with related coastal monitoring sensors; 4) integration with existing databases; and 5) internet capabilities with data streams to the World Wide Web. The potential for broader impacts includes immediate alignment with an existing CRCD project focused on creating a GIS-trained student able to use various data streams for accurate data synthesis modeling with a final capstone course combining both concepts into a real world situation. Further dissemination will occur through existing channels created by the RESSACA CREST and an existing REU Site. Texas A&M University-Corpus Christi is located in south Texas where the population demographics are heavily Hispanic, underrepresented groups will be provided opportunity to pursue advanced degrees in science and engineering as part of their research training and experience. The project will result in improved flow cytometry instrumentation that advances continuous monitoring of coastal margin environments, supporting research, education, and public policy designed to protect coastal margin ecosystems.