MRI: Development of An In-situ Flow Cytometer for Enhanced Aquatic Particle Discrimination

MRI：开发用于增强水生颗粒辨别的原位流式细胞仪

• 批准号: 0421310
• 负责人: James Bonner
• 金额: --
• 依托单位: Texas A&M Engineering Experiment Station
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421310&HistoricalAwards=false
• 关键词: MRI; Development; situ; Flow; Cytometer

Proposal No. CTS-0421310Principal Investigator: J. S. Bonner, Texas A&M University, Corpus ChristiThis grant will result in improved in situ flow cytometry instrumentation to enable real-time particle detection (colloidal, super-colloidal and microbial) in coastal margin environments. Coastal margins provide ecological and economic services of vast importance to society at large; advancing methods to ensure their sustainable management must continue to be a research and operational priority. It is widely recognized that real-time monitoring is needed for adequate detection of contaminants in coastal margin ecosystems. Furthermore, because of the complexity of interacting physical processes in these ecosystems, static monitoring systems are unable to resolve spatial variability at scales necessary for proper environmental management. Current flow cytometry technology allows for particle discrimination over time at a fixed location. It does not, however, support real-time detection over broader spatial scales. This is a critical limitation, and one that has hampered progress in protection of marine ecosystems from both natural and anthropogenic disturbances. The goal is to modify the commercial FlowCAM cytometer and its procedures to allow continuous marine monitoring over both space and time. A functional flow cytometer will be developed that can be housed in a tube approximately 9 inches in diameter and 3 feet in length. The instrument will be fitted for use on a towable platform for shallow marine environments. The modifications proposed here will allow: 1) quantitative aquatic particle differentiation; 2) rapid instrument deployment and enhanced mobility for use in shallow water environments; 3) synergy with related coastal monitoring sensors; 4) integration with existing databases; and 5) internet capabilities with data streams to the World Wide Web. The potential for broader impacts includes immediate alignment with an existing CRCD project focused on creating a GIS-trained student able to use various data streams for accurate data synthesis modeling with a final capstone course combining both concepts into a real world situation. Further dissemination will occur through existing channels created by the RESSACA CREST and an existing REU Site. Texas A&M University-Corpus Christi is located in south Texas where the population demographics are heavily Hispanic, underrepresented groups will be provided opportunity to pursue advanced degrees in science and engineering as part of their research training and experience. The project will result in improved flow cytometry instrumentation that advances continuous monitoring of coastal margin environments, supporting research, education, and public policy designed to protect coastal margin ecosystems.

建议书编号CTS-0421310首席研究员：J.S.Bonner，德克萨斯农工大学，Corpus Christian这笔赠款将改进原位流式细胞仪仪器，使沿海环境中的实时颗粒检测(胶体、超胶体和微生物)成为可能。沿海边缘提供对整个社会极为重要的生态和经济服务；改进方法以确保其可持续管理必须继续作为研究和业务优先事项。人们普遍认识到，需要进行实时监测，以便充分检测沿海边缘生态系统中的污染物。此外，由于这些生态系统中相互作用的物理过程的复杂性，静态监测系统无法在适当的环境管理所需的尺度上解决空间变异性。目前的流式细胞仪技术允许在固定位置随时间分辨颗粒。然而，它不支持在更大的空间尺度上进行实时检测。这是一个严重的限制，也阻碍了在保护海洋生态系统免受自然和人为干扰方面的进展。其目标是修改商用FlowCAM细胞仪及其程序，以允许在空间和时间上进行连续的海洋监测。将开发一种功能强大的流式细胞仪，它可以安装在直径约9英寸、长度约3英尺的管子中。该仪器将安装在浅海环境的拖曳平台上使用。这里提出的修改将允许：1)定量的水生颗粒差异；2)用于浅水环境的快速仪器部署和增强的机动性；3)与相关的沿海监测传感器的协同作用；4)与现有数据库的集成；以及5)互联网能力与流向万维网的数据流。潜在的更广泛的影响包括立即与现有的CRCD项目保持一致，该项目的重点是培养一名接受过地理信息系统培训的学生，能够使用各种数据流进行准确的数据合成建模，并通过最终的顶峰课程将这两个概念结合到现实世界中。将通过RESSACA CREST创建的现有渠道和现有的REU站点进行进一步传播。德克萨斯农工大学-科珀斯克里斯蒂大学位于得克萨斯州南部，那里的人口以西班牙裔为主，代表人数较少的群体将有机会攻读科学和工程方面的高级学位，作为他们研究、培训和经验的一部分。该项目将改进流式细胞仪仪器，促进对沿海边缘环境的持续监测，支持旨在保护沿海边缘生态系统的研究、教育和公共政策。