Functional Genomics of Maize Chromatin

玉米染色质的功能基因组学

• 批准号: 0421619
• 负责人: Candace Galen
• 金额: --
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421619&HistoricalAwards=false
• 关键词: Functional; Genomics; Maize; Chromatin

Chromatin plays a central role in gene expression during eukaryotic development and mediates epigenetic phenomena. Our goal is to determine the mechanisms that contribute to chromatin-based control of gene expression using maize, a premier model for these studies. It is becoming increasingly clear that understanding chromatin-level control of gene expression in plants cannot be extrapolated from other eukaryotes. Rather, direct experimentation in plants is needed. Understanding how chromatin controls gene expression in maize will provide new knowledge and molecular tools for precisely and effectively manipulating gene expression and phenotypes in this and other important crop plants. We will identify additional maize chromatin genes, including RNA-based silencing genes; complete a new set of chromatin mutants for maize (both RNA interference lines and recessive mutations); test function by assaying lines for 1) paramutation and epigenetic variegation; 2) transgene silencing; 3) loss of imprinting; 4) DNA methylation; 5) histone modification of repetitive elements; 6) changes in gene and transposon expression profiles; and 6) chromosome cytology. New vectors will enable high-throughput screens of cellular localization and protein-protein interactions. The combination of different types of functional data will provide an integrated picture of chromatin-level regulation of gene expression. This project will contribute to the education and training of students and postdoctoral students who will work in the participating research groups. In addition, plant genomics will be the focus of programs for science teachers and for undergraduate student interns. At the University of Arizona, high school teachers will join plant genomics labs for an 8-week summer program featuring individual lab work and development of genomics modules to use in their high school science classes. At the University of Missouri, science teachers will take part in a one-day workshop called "Chromatin: All Wrapped Up" as part of a year-long workshop series focusing on different aspects of plant genetics and genomics. The University of Missouri will also host undergraduate students from around the country to participate in 8-week summer internships as part of the Plant Genomics Internships @ MU program. All of these educational programs actively seek participants from underrepresented groups.Access to project outcomesAll resources will be available through the Maize Genetics Stock Cooperation (http://w3.aces.uiuc.edu/maize-coop/) and data will be available through The Plant Chromatin Database (ChromDB: http://www.chromdb.org/).

染色质在真核发育过程中在基因表达中起着核心作用，并介导表观遗传现象。我们的目标是确定使用玉米（这些研究的主要模型）对基于染色质控制基因表达的机制。 越来越清楚的是，了解植物中基因表达的染色质水平控制不能从其他真核生物中推断出来。相反，需要在植物中进行直接实验。 了解玉米中染色质如何控制基因表达将提供新知识和分子工具，以精确有效地操纵基因表达和该重要作物植物中的基因表达和表型。 我们将确定其他玉米染色质基因，包括基于RNA的沉默基因；完成一组新的玉米染色质突变体（RNA干扰线和隐性突变）；通过分析线的测试功能1）参数和表观遗传变化； 2）转基因沉默； 3）失去印迹； 4）DNA甲基化； 5）重复元素的组蛋白修饰； 6）基因和盆地表达谱的变化； 6）染色体细胞学。 新向量将使细胞定位和蛋白质 - 蛋白质相互作用的高通量筛选能够。不同类型的功能数据的组合将提供基因表达的染色质级调节的整合图片。该项目将有助于将在参与研究小组中工作的学生和博士后学生的教育和培训。 此外，植物基因组学将成为科学教师和本科生实习生计划的重点。 在亚利桑那大学，高中教师将加入Plant Genomics Labs，进行为期8周的夏季课程，其中包括个人实验室工作以及用于其高中科学课程的基因组学模块的开发。 在密苏里大学，科学教师将参加为期一天的研讨会，称为“染色质：全部包裹”，这是为期一年的研讨会系列的一部分，重点是植物遗传学和基因组学的各个方面。 密苏里大学还将在全国各地举办本科生，参加为期8周的暑期实习，这是植物基因组学实习 @ MU计划的一部分。 All of these educational programs actively seek participants from underrepresented groups.Access to project outcomesAll resources will be available through the Maize Genetics Stock Cooperation (http://w3.aces.uiuc.edu/maize-coop/) and data will be available through The Plant Chromatin Database (ChromDB: http://www.chromdb.org/).