Functional Genomics of Maize Chromatin

玉米染色质的功能基因组学

• 批准号: 0421619
• 负责人: Candace Galen
• 金额: --
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421619&HistoricalAwards=false
• 关键词: Functional; Genomics; Maize; Chromatin

Chromatin plays a central role in gene expression during eukaryotic development and mediates epigenetic phenomena. Our goal is to determine the mechanisms that contribute to chromatin-based control of gene expression using maize, a premier model for these studies. It is becoming increasingly clear that understanding chromatin-level control of gene expression in plants cannot be extrapolated from other eukaryotes. Rather, direct experimentation in plants is needed. Understanding how chromatin controls gene expression in maize will provide new knowledge and molecular tools for precisely and effectively manipulating gene expression and phenotypes in this and other important crop plants. We will identify additional maize chromatin genes, including RNA-based silencing genes; complete a new set of chromatin mutants for maize (both RNA interference lines and recessive mutations); test function by assaying lines for 1) paramutation and epigenetic variegation; 2) transgene silencing; 3) loss of imprinting; 4) DNA methylation; 5) histone modification of repetitive elements; 6) changes in gene and transposon expression profiles; and 6) chromosome cytology. New vectors will enable high-throughput screens of cellular localization and protein-protein interactions. The combination of different types of functional data will provide an integrated picture of chromatin-level regulation of gene expression. This project will contribute to the education and training of students and postdoctoral students who will work in the participating research groups. In addition, plant genomics will be the focus of programs for science teachers and for undergraduate student interns. At the University of Arizona, high school teachers will join plant genomics labs for an 8-week summer program featuring individual lab work and development of genomics modules to use in their high school science classes. At the University of Missouri, science teachers will take part in a one-day workshop called "Chromatin: All Wrapped Up" as part of a year-long workshop series focusing on different aspects of plant genetics and genomics. The University of Missouri will also host undergraduate students from around the country to participate in 8-week summer internships as part of the Plant Genomics Internships @ MU program. All of these educational programs actively seek participants from underrepresented groups.Access to project outcomesAll resources will be available through the Maize Genetics Stock Cooperation (http://w3.aces.uiuc.edu/maize-coop/) and data will be available through The Plant Chromatin Database (ChromDB: http://www.chromdb.org/).

染色质在真核生物发育过程中的基因表达中起着核心作用，并介导表观遗传现象。我们的目标是确定的机制，有助于染色质为基础的控制基因的表达，使用玉米，这些研究的首要模型。 越来越清楚的是，理解植物中基因表达的染色质水平控制不能从其他真核生物中推断。相反，需要在植物中进行直接实验。 了解染色质如何控制玉米中的基因表达，将提供新的知识和分子工具，精确和有效地操纵基因表达和表型在这个和其他重要的作物植物。 我们将鉴定更多的玉米染色质基因，包括基于RNA的沉默基因;完成一套新的玉米染色质突变体（RNA干扰系和隐性突变）;通过测定系的1）副突变和表观遗传杂色; 2）转基因沉默; 3）印记丢失; 4）DNA甲基化; 5）重复元件的组蛋白修饰; 6）基因和转座子表达谱的变化;和6）染色体细胞学。 新的载体将使细胞定位和蛋白质-蛋白质相互作用的高通量筛选成为可能。不同类型的功能数据的组合将提供基因表达的染色质水平调控的综合图片。该项目将有助于教育和培训将在参与研究小组工作的学生和博士后学生。 此外，植物基因组学将成为科学教师和本科生实习生项目的重点。 在亚利桑那大学，高中教师将加入植物基因组学实验室，参加为期8周的暑期课程，其中包括个人实验室工作和基因组学模块的开发，以用于他们的高中科学课程。 在密苏里州大学，科学教师将参加一个为期一天的研讨会，名为“染色质：所有包裹起来”，作为为期一年的研讨会系列的一部分，重点是植物遗传学和基因组学的不同方面。 密苏里州大学还将接待来自全国各地的本科生参加为期8周的暑期实习，作为植物基因组学实习@ MU计划的一部分。 所有这些教育项目都积极寻求来自代表性不足群体的参与者。获得项目成果所有资源都将通过玉米遗传学股份合作公司（http：//w3.aces.uiuc.edu/maize-coop/）获得，数据将通过植物染色质数据库（ChromDB：http：//www.chromdb.org/）获得。