Phylogenetic Analysis of Eurasion Allotetraploid Species of Elymus (Gramineae) Based on Four Molecular Data Sets.

基于四个分子数据集的披碱草（禾本科）Eurasion异源四倍体物种的系统发育分析。

• 批准号: 0426194
• 负责人: Roberta Mason-Gamer
• 金额: --
• 依托单位: University of Illinois at Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0426194&HistoricalAwards=false
• 关键词: Phylogenetic; Analysis; Eurasion; Allotetraploid; Species

0426194Mason-Gamer Research by Dr. Roberta Mason-Gamer of the University of Illinois-Chicago is designed to clarify the evolutionary history of several European and Asian species in the grass genus Elymus. Elymus is classified within the wheat tribe, Triticeae, along with wheat, barley, and rye. From an evolutionary standpoint, Elymus is interesting because of its complex genetic history. While the genetically simplest, or diploid, members of the Triticeae have two complete sets of chromosomes (one set from each parent, just as in humans), Elymus individuals have four, six, or even eight complete sets, representing the combined genomes from two or more genetically and evolutionarily distinct ancestors. This research focuses on the four-genome (tetraploid) members of Elymus that are native to Europe and Asia, and builds directly upon earlier research on the tetraploid Elymus species of North America. The objective of the research is to clarify the evolutionary history of 18 of these complex Eurasian species using DNA sequence data from four different molecular markers. More specifically, the goals are to: (1) identify the diploid maternal and paternal progenitors of each species, and (2) assess whether patterns of genetic diversity within Elymus correspond either to broad geographic patterns, or to previously observed patterns of outwardly visible morphological variation. When data from these species are combined with existing data from the North American tetraploids, this research will encompass the largest group of polyploids examined to date using detailed molecular phylogenetic methods. Studies of polyploids are relevant to understanding the extraordinary diversity of flowering plants on earth, since as many as half of all flowering plant species are of polyploid origin. Not only does polyploidy represent a major mechanism of plant speciation, but some of our most important crop plants are polyploid, including alfalfa, canola, cotton, coffee, cruciferous vegetables, potatoes, soybeans, tobacco, wheat, and many others. Thus, mechanisms of polyploid formation and the genetic changes that accompany polyploidy are of broad interest to plant scientists in many fields of research. Evolutionary studies such as the one described here lay the necessary groundwork for detailed explorations of many other facets of polyploidy. In addition to providing insights into the complicated history of Elymus, the project will support student education at the University of Illinois at Chicago in two ways. First, two graduate students will be supported by the grant. They will be deeply involved with the collection, analysis, and interpretation of genetic data. Whatever they gain from this experience in terms of methods and ideas will directly enrich their own thesis research. Second, undergraduate student participation will be an important part of completing the project. The grant provides work-study wages, and also supports the cost of supplies for students who wish to receive course credit for completing research projects. Undergraduates will learn many broadly applicable laboratory techniques, including DNA isolation, gene amplification, and DNA sequencing.

0426194 Mason-Gamer伊利诺伊大学芝加哥分校的Roberta Mason-Gamer博士的研究旨在澄清欧洲和亚洲几种草属Escherichus的进化历史。 Escherichus与小麦、大麦和黑麦沿着被分类在小麦族（Triticeae）中。 从进化论的角度来看，伊克诺斯是有趣的，因为它复杂的遗传历史。 虽然遗传上最简单的小麦族成员或二倍体有两套完整的染色体（一套来自每个父母，就像人类一样），但Escherichus个体有四套，六套甚至八套完整的染色体，代表来自两个或更多遗传和进化上不同的祖先的组合基因组。 这项研究的重点是原产于欧洲和亚洲的四基因组（四倍体）成员，并直接建立在早期对北美四倍体Escherichus物种的研究基础上。 这项研究的目的是利用来自四种不同分子标记的DNA序列数据来阐明这些复杂的欧亚物种中的18种的进化历史。 更具体地说，目标是：（1）确定每个物种的二倍体母本和父本祖先，（2）评估是否模式的遗传多样性在Escherichus内对应于广泛的地理模式，或以前观察到的模式的外观可见的形态变异。 当这些物种的数据与北美四倍体的现有数据相结合时，这项研究将包括迄今为止使用详细的分子系统发育方法研究的最大一组多倍体。对多倍体的研究有助于了解地球上开花植物的非凡多样性，因为所有开花植物物种中有多达一半是多倍体起源的。 多倍体不仅代表了植物物种形成的主要机制，而且我们一些最重要的作物也是多倍体，包括苜蓿、油菜、棉花、咖啡、十字花科蔬菜、马铃薯、大豆、烟草、小麦和许多其他植物。 因此，多倍体形成的机制和伴随多倍体的遗传变化是植物科学家在许多研究领域的广泛兴趣。 进化的研究，如这里所描述的一个奠定了必要的基础，详细探索许多其他方面的多倍性。 除了提供对埃克塞特复杂历史的见解外，该项目还将以两种方式支持伊利诺伊大学芝加哥分校的学生教育。 首先，两名研究生将得到赠款的支持。 他们将深入参与遗传数据的收集，分析和解释。 他们从中获得的方法和思想上的收获，将直接丰富自己的论文研究。 第二，本科生的参与将是完成项目的重要组成部分。 该补助金提供勤工俭学工资，并支持那些希望获得课程学分以完成研究项目的学生的用品费用。 本科生将学习许多广泛适用的实验室技术，包括DNA分离，基因扩增和DNA测序。