Sensors: Immobilization Technology for Array-based Biosensors

表面等离子体共振生物传感器使分子间相互作用的研究发生了革命性的变化。目前的仪器无需试剂标记即可提供复杂地层的详细动力学信息，但处理能力较低。新的基于阵列的生物传感器技术将通过增加可以同时监测的表面反应的数量，为蛋白质组学革命做出重大贡献。生产蛋白质阵列的挑战有两个：将活性蛋白质沉积并保持在表面上，以及在小的靶区(通常为1平方厘米)内物理地制造密集的蛋白质阵列。目前的奖项旨在优化多肽的固相定位条件，并制造允许流动蛋白质固定化的新型流体设备。这项工作的更广泛影响包括培训学生在生物传感器技术和核受体生物学方面的知识。此外，这项工作产生的技术将允许大规模绘制蛋白质相互作用图，这将作为解释复杂生物系统的基础。

ABSTRACTSurface plasmon resonance biosensors have revolutionized the study ofmolecular interactions. Current instruments can provide detailed kineticinformation about complex formation without reagent labeling, but arelow throughput. New array-based biosensor technology is poised tomake a significant contribution to the proteomics revolution by increasing the number of surface reactions that can be monitored simultaneously. Thechallenges in producing protein arrays are two fold: depositing and maintainingactive proteins on a surface and physically creating dense arrays of proteins within small target areas (typically 1 cm2). The current award seeks to optimize the conditions for solid-pin spotting of peptides and to fabricate novel fluidic devices that would allow for in-flow protein immobilization.The broader impacts of this work include training students in biosensor technologyand nuclear receptor biology. In addition, the technology that results from this workwill permit large-scale mapping of protein interactions, which will serve as thebasis for interpreting complex biological systems.