Surface Plasmon Resonance Biosensor Data Processing and Analysis Software

该合同支持改进数据处理和分析软件的开发，用于表面等离子体共振（SPR）生物传感器。在过去的10年里，SPR传感器的使用已经成为测量生物分子相互作用的标准方法，如那些涉及抗体、受体、酶和寡核苷酸的相互作用。目前可用的许多工具都是自动化的，因此能够生成关于交互的大量信息。然而，目前还没有可用于处理生物传感器数据的自动化程序，使用现有的手动评估软件既繁琐又耗时。本项目的目标是开发新的软件，旨在将处理生物传感器数据所需的时间从几小时减少到几秒钟，同时，通过使用新颖的参考技术显着提高数据质量。计划进一步发展将扩展生物传感器在活性浓度测定领域的能力。专门的误差空间分析程序也将被纳入，以提供有关生物传感器分析结果的详细信息。新的软件，以及教育手册和教程，将通过互联网免费提供。使用这种自动数据处理软件将把实时生物传感器分析的潜力变为现实。

This award supports the development of improved Data Processing and Analysis Software to be used with surface plasmon resonance (SPR) biosensors. In the last 10 years, the use of SPR sensors have become a standard method for measuring biomolecular interactions such as those involving antibodies, receptors, enzymes, and oligonucleotides. Many of the instruments that are available today are automated and, therefore, capable of generating a wealth of information about an interaction. However, there are currently no automated programs available to process biosensor data, and the use of available manual evaluation software is tedious and time consuming The goal of this project is the development of new software designed to reduce the time required for processing biosensor data from hours to seconds, while at the same time, significantly improving the quality of data through the use of novel referencing techniques. Additional developments are planned that will extend the capabilities of biosensors in the area of active concentration determination. Specialized error space analysis routines will also be incorporated to provide detailed information about the results of biosensor analysis. The new software, as well as educational manuals and tutorials, will be made freely available through the Internet. Access to this automated data processing software will turn the potential of real-time biosensor analysis into a reality.