SENSORS: Cell-Based Sensors that link Nanotechnology with Microfluidics

这个项目将专门开发一个原始的微流体网络(USTAR)来研究多价纳米传感器对基本细胞趋化迁移过程的影响。该系统一旦被诱导到可存活的成纤维细胞内，将能够对纳米传感器动力学进行单细胞成像和定量分析。将开发纳米传感器来靶向细胞内蛋白，生长因子受体结合-2(Grb2)，已知当被本项目研究的趋化生长因子激活时，启动趋化信号级联反应。

0428573VazquezThis project will specifically develop an original microfluidic network (the uStar) to study the effects of multivalent nanosensors on the fundamental cell process of chemotaxic migration. The system will enable single-cell imaging and quantitative analysis of nanosensor dynamics once inducted within viable fibroblast cells in real-time. Nanosensors will be developed to target the intracellular protein, Growth Factor Receptor Bound-2 (GRB2), known to initiate chemotaxis signaling cascades when activated by the chemoattractant growth factors studied in this project.