Collaborative Project - Time/space Patterns of Microsatellite DNA Variation in Relation to Patch Structure of Southern Ocean Krill, Euphasia Superba

拟议的研究将使用遗传标记来检查南大洋磷虾的种群遗传多样性和结构。这项工作建立在以前的工作，由一个项目的参与者，以开发这些遗传标记和适当的协议，为E。superba。计划对2001年和2002年在南大洋考察南极洲西部半岛期间收集的存档样本进行分子遗传分析。遗传多样性和结构的时间和空间模式将在磷虾群，水深的收集区，和中尺度海洋环流的背景下进行描述。E.将从用酒精保存的浮游动物样本中识别出superba。来自不同巡航和不同区域的磷虾将被用来筛选20至40个遗传标记，以确定在南大洋不同区域和一年中不同时间收集的磷虾之间的遗传差异水平。这项工作的长期目标是促进我们对决定南大洋磷虾的补充和维持的种群动态过程的理解。这一努力的更广泛影响包括培训一名本科生;与欧洲的研究人员和学生进行研究和教育方面的国际合作;以及对可能受到全球变化影响的极地生态系统中关键物种的遗传结构的新认识。

The proposed study will use genetic markers to examine population genetic diversity and structure of the Southern Ocean krill, Euphausia superba (Crustacea, Euphausiacea). This effort builds upon previous work by one of the project participants to develop these genetic markers and the appropriate protocols for E. superba. Molecular genetic analysis is planned for archived samples collected during Southern Ocean cruises to the Western Antarctic Peninsula (WAP) during 2001 and 2002. Temporal and spatial patterns of genetic diversity and structure will be described in the context of krill swarms, bathymetry of the collection region, and mesoscale ocean circulation. Specimens of E. superba will be identified from alcohol-preserved zooplankton samples. Krill from different cruises and diverse regions of the sampled domain will be used to screen 20 to 40 genetic markers to determine levels of genetic differences between krill collected in different regions and at different times of the year in the Southern Ocean. The long-term goal of this effort is to contribute to our understanding of the population dynamic processes determining recruitment and maintenance of Southern Ocean krill. Broader impacts for this effort include training of an undergraduate student; international collaboration in research and education with researchers and students in Europe; and new understanding of the genetic architecture of a pivotal species in a polar ecosystem that is likely to be impacted by global change.