Collaborative Project - Time/space Patterns of Microsatellite DNA Variation in Relation to Patch Structure of Southern Ocean Krill, Euphasia Superba

这项拟议的研究将使用遗传标记来研究南大洋磷虾Euphausia Superba(甲壳纲、Euphausiacea)的种群遗传多样性和结构。这项工作建立在一个项目参与者之前的工作基础上，为E.Superba开发这些遗传标记和适当的协议。计划对2001年至2002年期间南大洋游轮前往南极半岛西部(WAP)期间收集的存档样本进行分子遗传分析。遗传多样性和结构的时间和空间模式将在磷虾群、采集区的水深测量和中尺度海洋环流的背景下描述。超级E.Superba的标本将从酒精保存的浮游动物样本中鉴定出来。将使用来自采样区域不同游轮和不同区域的磷虾筛选20至40个遗传标记，以确定在不同区域和南大洋不同时间收集的磷虾之间的遗传差异程度。这一努力的长期目标是促进我们对决定南大洋磷虾的招募和维持的种群动态过程的了解。这一努力的更广泛影响包括培训一名本科生；与欧洲的研究人员和学生在研究和教育方面进行国际合作；以及对极地生态系统中可能受到全球变化影响的关键物种的遗传结构有新的理解。

The proposed study will use genetic markers to examine population genetic diversity and structure of the Southern Ocean krill, Euphausia superba (Crustacea, Euphausiacea). This effort builds upon previous work by one of the project participants to develop these genetic markers and the appropriate protocols for E. superba. Molecular genetic analysis is planned for archived samples collected during Southern Ocean cruises to the Western Antarctic Peninsula (WAP) during 2001 and 2002. Temporal and spatial patterns of genetic diversity and structure will be described in the context of krill swarms, bathymetry of the collection region, and mesoscale ocean circulation. Specimens of E. superba will be identified from alcohol-preserved zooplankton samples. Krill from different cruises and diverse regions of the sampled domain will be used to screen 20 to 40 genetic markers to determine levels of genetic differences between krill collected in different regions and at different times of the year in the Southern Ocean. The long-term goal of this effort is to contribute to our understanding of the population dynamic processes determining recruitment and maintenance of Southern Ocean krill. Broader impacts for this effort include training of an undergraduate student; international collaboration in research and education with researchers and students in Europe; and new understanding of the genetic architecture of a pivotal species in a polar ecosystem that is likely to be impacted by global change.