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Functions of mycorrhizhosphere communities under the influence of different nitrogen and water regimes in forest soils

森林土壤不同氮水状况影响下菌根际群落的功能

基本信息

批准号：
168309971
负责人：
Professor Dr. Michael Schloter
金额：
--
依托单位：
Institut für Biochemische Pflanzenpathologie (BIOP)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2013-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/168309971?language=en
关键词：
Functions mycorrhizhosphere communities under influence

项目摘要

It will be the aim of this project to understand the close interplay between plant performance and microbial activity in the rhizosphere of drought stressed beech trees. We postulate that due to reduced assimilation of the plants and increased expression of secondary metabolites, the quality and quantity of exudates is changed compared to unstressed trees. This will affect microbial community structure and functions including N transformation and carbon cycling in the rhizosphere. We postulate that while the microbial communities in the rhizosphere of non stressed trees are characterized high activity pattern and mainly hydrolytic functions enabling them to metabolize easily degradable substances like carbohydrates and amino acids, the rhizosphere of stressed trees is shifted towards low activity and microbes which are capable to express oxidative enzymes enabling them to get access to nutrients entrapped in phenolic polymers such as humic acids and lignin. This shift changes in microbial performance will also affect nitrogen turnover, as mainly expression pattern of genes involved in mineralization and denitrification activities will be changed. To investigate this hypothesis, molecular tools (microarray analysis) of microbial community structure and function and biochemical enzymatic tests will be used. Furthermore, specific enzymes in the nitrogen cycle will be studied using real-time quantitative PCR.

这将是本项目的目的是了解植物性能和微生物活动之间的密切相互作用，在干旱胁迫的山毛榉树的根际。我们假设，由于减少同化的植物和次生代谢产物的表达增加，分泌物的质量和数量的变化相比，无压力的树木。这将影响根际微生物群落结构和功能，包括氮转化和碳循环。我们推测，虽然非胁迫树木根际微生物群落的特征是高活性模式和主要水解功能，使它们能够代谢易降解的物质，如碳水化合物和氨基酸，受胁迫树木的根际向低活性和能够表达氧化酶的微生物转移，使它们能够获得捕获在酚聚合物如腐殖酸中的营养物和木质素。微生物性能的这种转变也会影响氮素周转，因为主要参与矿化和反硝化活动的基因的表达模式将发生变化。为了研究这一假设，将使用微生物群落结构和功能的分子工具（微阵列分析）以及生化酶试验。此外，将使用实时定量PCR研究氮循环中的特定酶。