Driver of microbial nutrient turnover in mineral soil, rhizosphere and forest floors

矿质土壤、根际和森林地面微生物养分周转的驱动因素

基本信息

项目摘要

The forest floor (FF) is the boundary between mineral soil and the atmosphere and is thus important for nutrient transport from the surface to the mineral soil. FF properties influence nutrient concentrations and quality as well as oxygen and water availability. The turnover of nutrients by microorganisms is regulated on the level of nutrient stoichiometry of ecosystems and genetic operon structure of single microorganisms who pursue different strategies to regulate efficient nutrient use. Thus, nutrient availability and community composition are closely interlinked. Consequently, changes in nutrient availability and habitat structure because of increasing temperature feedback on microbial communities in terms of their taxonomic and functional composition as well as trophic interactions. The aim of our project is to understand the interplay of abiotic FF properties with the microbial community composition under different P and temperature regimes. We hypothesize that the quality and quantity of soil organic matter and root exudates as well as nutrient stoichiometry (C, N, P, cations) drives the microbial potential to transform nitrogen and phosphorus and determines trophic interaction with other biota (fungi, fauna and trees). Moreover, spatial correlation of bacteria and nutrient hotspots of N and P are likely. To test these overarching hypotheses we will use a combination of experimental and analytical approaches. We will first identify key players and processes of microbial N and P turnover. Therefore, we will take samples in different FF layers and the mineral soil from 12 beech dominated sites displaying gradients of P concentrations and T and in the rhizosphere of beech, maple and spruce from three sites with contrasting P availability. To identify the long-term adaptation of microbial communities to FF properties and tree species we will combine metagenomics approaches for the identification of key processes and players and subsequent qPCR for the quantification of dominant key players under different settings. Moreover, we will investigate the colonization of roots by microbes involved in P turnover in FF and mineral soil by using CARD-FISH. Second, we will use different experimental approaches: (i) To identify the role of soil minerals for P availability and microbial P acquisition strategies in frame of a litter/OF bag experiment where additional primary and secondary minerals will be added to litter or OF material. (ii) To disentangle the interplay of FF fauna and microorganisms in frame of a fauna exclusion experiment. In addition to the developed quantification tools, we will also isolate bacteria involved in the solubilisation of mineral bound P of the litter/OF bag experiment to reconstruct operon structures of P genes. Based on the different approaches we will be able to identify major drivers of microbial community composition and derived services important for FF turnover under changing environmental conditions.
森林地面(FF)是矿质土壤和大气之间的边界,因此对于从地表到矿质土壤的养分运输是重要的。FF的特性影响养分浓度和质量以及氧气和水分的有效性。微生物的养分周转受生态系统的营养化学计量水平和单个微生物的遗传操纵子结构的调节,这些微生物追求不同的策略来调节有效的养分利用。因此,养分有效性与群落组成密切相关。因此,由于温度升高,营养物有效性和生境结构的变化在微生物群落的分类和功能组成以及营养相互作用方面反馈。我们项目的目的是了解在不同P和温度条件下非生物FF特性与微生物群落组成的相互作用。我们假设土壤有机质和根系分泌物的质量和数量以及养分化学计量(C, N, P,阳离子)驱动微生物转化氮和磷的潜力,并决定与其他生物群(真菌,动物和树木)的营养相互作用。细菌与氮、磷养分热点之间存在一定的空间相关性。为了验证这些总体假设,我们将使用实验和分析方法相结合的方法。我们将首先确定微生物N和P周转的关键参与者和过程。因此,我们将在不同FF层和矿质土壤中采集样品,从12个山毛榉为主的地点采集样品,显示P浓度和T的梯度,并在三个不同地点的山毛榉、枫和云杉根际取样,对比P有效性。为了确定微生物群落对FF特性和树种的长期适应,我们将结合宏基因组学方法来识别关键过程和参与者,并随后结合qPCR来量化不同环境下的优势关键参与者。此外,我们将利用CARD-FISH研究参与磷转化的微生物在FF和矿质土壤中的根定植。其次,我们将使用不同的实验方法:(i)在凋落物/ of袋实验的框架下,确定土壤矿物质对磷有效性和微生物磷获取策略的作用,其中将额外的初级和次级矿物质添加到凋落物或of材料中。(ii)在动物群排斥实验的框架内解开FF动物群和微生物的相互作用。除了开发的定量工具外,我们还将分离凋落物/ of袋实验中参与矿物结合P溶解的细菌,以重建P基因的操纵子结构。基于不同的方法,我们将能够确定微生物群落组成的主要驱动因素以及在不断变化的环境条件下对FF周转重要的衍生服务。

项目成果

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Professor Dr. Michael Schloter其他文献

Professor Dr. Michael Schloter的其他文献

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{{ truncateString('Professor Dr. Michael Schloter', 18)}}的其他基金

PriOrity Effect Mechanisms (POEM): mechanisms of priority effects and their persistence over time in dry acidic grasslands
优先效应机制(POEM):优先效应机制及其在干燥酸性草原中随时间的持续性
  • 批准号:
    420444099
  • 财政年份:
    2019
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Linking carbon and nitrogen availability to microbial phosphorous turnover in different forest soils
将不同森林土壤中碳和氮的有效性与微生物磷周转联系起来
  • 批准号:
    240847006
  • 财政年份:
    2013
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Effects of veterinary medicines on the functional diversity of the microbial biomass in different soil compartments in dependence of varying soil moisture conditions
不同土壤湿度条件下兽药对不同土壤区划微生物量功能多样性的影响
  • 批准号:
    201573247
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
    Research Units
Functions of mycorrhizhosphere communities under the influence of different nitrogen and water regimes in forest soils
森林土壤不同氮水状况影响下菌根际群落的功能
  • 批准号:
    168309971
  • 财政年份:
    2010
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Dynamics of microbial communities involved in carbon and nitrogen turnover during paddy soil evolution in relation to different soil types
不同土壤类型水稻土演化过程中参与碳氮周转的微生物群落动态
  • 批准号:
    60228629
  • 财政年份:
    2008
  • 资助金额:
    --
  • 项目类别:
    Research Units
Land use intensity as a driver for abundance, diversity and activity of nitrifying microbes in soils from forest ecosystems
土地利用强度是森林生态系统土壤中硝化微生物丰度、多样性和活性的驱动因素
  • 批准号:
    61314588
  • 财政年份:
    2008
  • 资助金额:
    --
  • 项目类别:
    Infrastructure Priority Programmes
Effects of veterinary medicines on the functional diversity of the microbial biomass in soils
兽药对土壤微生物量功能多样性的影响
  • 批准号:
    5446265
  • 财政年份:
    2005
  • 资助金额:
    --
  • 项目类别:
    Research Units
SP08: Microbiome and stress
SP08:微生物组和压力
  • 批准号:
    432651233
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
    Research Units

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碳-铁-微生物对滩涂围垦稻田土壤团聚体形成和稳定的调控机制
  • 批准号:
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    2007
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    青年科学基金项目

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Mi-Hy - Microbial Hydroponics for nutrient recovery and utilisation
Mi-Hy - 用于养分回收和利用的微生物水培法
  • 批准号:
    10079655
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Gatekeeping glycan metabolism in the human gut microbiome
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