Effects of veterinary medicines on the functional diversity of the microbial biomass in different soil compartments in dependence of varying soil moisture conditions

不同土壤湿度条件下兽药对不同土壤区划微生物量功能多样性的影响

• 批准号: 201573247
• 负责人: Professor Dr. Michael Schloter
• 金额: --
• 依托单位: Institut für Pflanzenökologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/201573247?language=en
• 关键词: Effects; veterinary; medicines; functional; diversity

We observed in the 2nd phase that changing soil moisture conditions affected the antibiotic SDZ impact on abundance and diversity of functional microbial communities and enzymatic nitrogen turnover processes in soil. However, it was not possible to estimate to what extent the varying soil water contents were responsible for the observations. It seems indeed to be essential to bridge the gap of knowledge about the consequences of variable soil moisture for (fate and) effect of antibiotics being routinely introduced to agricultural soils, since considerable, natural variations in soil water contents frequently occur under field conditions. The objective of subproject B2 for the proposed final 3rd phase is therefore to investigate the influence of soil drying and rewetting on the effect of SDZ on the nitrogen cycling enzymatic activities and the respective abundance and diversity of functional microbial communities. To this end, B2 will perform a central climate chamber experiment under constant temperature conditions using SDZ-containing and non-contaminated pig manure encompassing two treatments: (i) constant soil water content (control) and (ii) drying-rewetting cycles. This central laboratory experiment will complement the Central Field Experiment with different soil moisture conditions and repeated applications of manure, in which soil moisture and temperature cannot be manipulated independently. Using the established methods we will investigate rhizosphere soil, rhizoplane and endorhizosphere and will additionally determine the diversity of the functional microbial via high throughput sequencing.

在第二阶段，我们观察到土壤水分条件的变化影响抗生素SDZ对土壤中功能性微生物群落的丰度和多样性以及酶促氮周转过程的影响。然而，无法估计不同的土壤含水量在多大程度上对观察结果负责。这似乎确实是必不可少的桥梁差距的知识变化的土壤水分的后果（命运和）的影响，抗生素被常规引入农业土壤，因为相当大的，自然变化的土壤含水量经常发生在田间条件下。因此，拟议的最后第三阶段的子项目B2的目标是调查土壤干燥和再湿润对SDZ对氮循环酶活性和功能微生物群落各自丰度和多样性的影响。为此，B2将在恒温条件下使用含SDZ和未受污染的猪粪进行中央气候室实验，包括两种处理：（i）恒定土壤含水量（对照）和（ii）干燥-再润湿循环。这一中心实验室实验将补充中心田间实验，其中不同的土壤湿度条件和重复施用粪肥，土壤湿度和温度不能单独操纵。使用已建立的方法，我们将调查根际土壤，根面和内围，并将通过高通量测序另外确定功能微生物的多样性。

项目成果

Manure contaminated with the antibiotic sulfadiazine impairs the abundance of nirK- and nirS-type denitrifiers in the gut of the earthworm Eisenia fetida

被抗生素磺胺嘧啶污染的粪便会损害蚯蚓 Eisenia fetida 肠道中 nirK 和 nirS 型反硝化菌的丰度

• DOI: 10.1007/s00374-009-0434-3
• 发表时间: 2010
• 期刊: Biology and Fertility of Soils
• 影响因子: 6.5
• 作者: Kotzerke;Kleineidam;Schloter
• 通讯作者: Schloter

Influence of difloxacin-contaminated manure on microbial community structure and function in soils

• DOI: 10.1007/s00374-010-0517-1
• 发表时间: 2011-02
• 期刊: Biology and Fertility of Soils
• 影响因子: 6.5
• 作者: Anja Kotzerke;Ute Hammesfahr;K. Kleineidam;M. Lamshöft;S. Thiele-Bruhn;M. Schloter;B. Wilke

Persistence of the fluoroquinolone antibiotic difloxacin in soil and lacking effects on nitrogen turnover.

• DOI: 10.2134/jeq2011.0459
• 发表时间: 2012-07
• 期刊: Journal of environmental quality
• 影响因子: 2.4
• 作者: Ingrid Rosendahl;J. Siemens;R. Kindler;J. Groeneweg;J. Zimmermann;S. Czerwinski;M. Lamshöft;V. Laabs;B. Wilke;H. Vereecken;W. Amelung

Effect of Sulfadiazine-Contaminated Pig Manure on the Abundances of Genes and Transcripts Involved in Nitrogen Transformation in the Root-Rhizosphere Complexes of Maize and Clover

• DOI: 10.1128/aem.01252-10
• 发表时间: 2010-10
• 期刊: Applied and Environmental Microbiology
• 影响因子: 4.4
• 作者: Julien F. Ollivier;K. Kleineidam;Rüdiger Reichel;S. Thiele-Bruhn;Anja Kotzerke;R. Kindler;B. Wilke