CAREER: Macromolecular Recognition and Differential Ion Channel Functioning

职业：大分子识别和差异离子通道功能

• 批准号: 0447541
• 负责人: Lisa Gentile
• 金额: --
• 依托单位: Western Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-15 至 2007-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0447541&HistoricalAwards=false
• 关键词: CAREER; Macromolecular; Recognition; Differential; Ion

This project describes plans for the continued development of a high quality research program in protein biophysical chemistry, and its integration into an educational plan involving high school, undergraduate, and master's students, as well as high school chemistry and biology teachers. The intellectual merit of this research focuses on understanding the underlying molecular recognition of a class of sulfated steroids that inversely modulate ion channels belonging to the ionotropic glutamate receptor (iGluR) family. Not only will this knowledge further the understanding of molecular recognition, but it will also aid in the ability to decipher the complex functioning of the brain. What function do these neurosteroids play in regulating our memory, cognition, and learning? The educational component integrates high school chemistry and biology teachers along with their students into the above research plan. The specific aims are to:1. Map the binding site of pregnenolone sulfate (PREGS) and pregnanolone sulfate (PREGAS) to the iGluRs and understand why PREGS is a positive regulator of the NMDA receptor, but a negative regulator of the AMPA receptor.2. Involve high school chemistry and biology teachers in an inquiry based investigation, which will enable them to develop new techniques, gather ideas to incorporate into new courses or labs, and satisfy their clock hour requirements.3. Involve high school juniors and seniors in a full time summer research program. This will allow these students to develop critical thinking and problem solving skills, as well as to expose them to the excitement of science and the possibility of future careers in the sciences.The broader impacts of this project are primarily in addressing scientific literacy. Not only will undergraduates and high school students be trained by this program, but also the high school teachers will participate in this program. Involvement of teachers in a research-based inquiry will aid in their ability to teach the process of science to students in a highly effective manner.

本项目描述了蛋白质生物物理化学高质量研究项目的持续发展计划，并将其整合到涉及高中、本科生和硕士生以及高中化学和生物教师的教育计划中。本研究的智力价值集中在理解一类硫酸类固醇的潜在分子识别，这些类固醇可反向调节属于嗜离子性谷氨酸受体（iGluR）家族的离子通道。这些知识不仅将进一步加深对分子识别的理解，而且还将有助于破译大脑复杂功能的能力。这些神经类固醇在调节我们的记忆、认知和学习方面起什么作用？教育部分将高中化学和生物教师及其学生纳入上述研究计划。具体目标是：1。将硫酸孕烯醇酮（PREGS）和硫酸孕烯醇酮（PREGAS）的结合位点映射到iGluRs上，并了解为什么PREGS是NMDA受体的正调节因子，而AMPA受体的负调节因子。让高中化学和生物教师参与以探究为基础的调查，这将使他们能够开发新技术，收集想法，将其纳入新的课程或实验中，并满足他们的工作时间要求。让高中三年级和四年级的学生参加一个全日制的暑期研究项目。这将使这些学生培养批判性思维和解决问题的能力，并使他们接触到科学的兴奋和未来科学职业的可能性。这个项目更广泛的影响主要是解决科学素养问题。该项目不仅对本科生和高中生进行培训，还将对高中教师进行培训。教师参与以研究为基础的探究将有助于他们以高效的方式向学生教授科学过程的能力。