SBIR Phase II: Microbial Enhancement of Soybeans for Salmonid Diets

该小型企业创新研究（SBIR）第二阶段项目旨在开发一种提高大豆营养价值的工艺，以取代鱼粉作为养殖鳟鱼和鲑鱼饲料的主要成分。 鱼粉对水产养殖业造成环境和经济限制。植物源蛋白是一种很好的替代饲料来源，但不能满足许多养殖鱼类（包括鳟鱼和鲑鱼）的营养需求。 之前的第一阶段工作表明，将选定的真菌菌株与固体基质培养（SSC）的创新相结合，将增加大豆中的蛋白质含量，消除不可消化的碳水化合物，并减少抗营养因子。 第二阶段项目将测试中试规模的SSC技术，以确定生产生物增强大豆蛋白的商业过程的工程设计和经济性，并在鳟鱼饲养试验中证明这种蛋白质的饲料价值。该项目的商业应用将在水产养殖业。鱼粉的使用给养鱼户带来了经济、市场和水污染问题，消费者也对环境影响感到担忧（例如，有报告称，在喂食鱼粉的养殖鲑鱼中检测到较高水平的多氯联苯、二恶英和其他农药）。用植物蛋白替代鱼粉将通过增加鱼类消费促进健康，并将缓解水产养殖业面临的环境和经济限制。

This Small Business Innovation Research (SBIR) Phase II project aims to develop a process for enhancing the nutritional value of soybeans to replace fishmeal as the primary ingredient in farmed trout and salmon feed. Fishmeal creates environmental and economic constraints for the aquaculture industry. Plant-derived proteins are a good alternate feed source, but do not meet the nutritional requirements of many farmed fish species including trout and salmon. Prior Phase I work demonstrated that a combination of a selected fungal strain with innovations in solid substrate culture (SSC) would increase the protein content, eliminate the non-digestible carbohydrates and reduce anti-nutritional factors in soybeans. This Phase II project will test pilot-scale SSC technology to determine engineering design and economics for a commercial process to manufacture the bio-enhanced soy protein, and to demonstrate the feed value of this protein in trout feeding trials. The commercial application of this project will be in the aquaculture industry. The use of fishmeal creates economic, market and water pollution issues for fish farmers, and consumer concerns regarding environmental impacts (for example, there are reports of PCBs, dioxins, and other pesticides detected at higher levels in farmed salmon that have been fed fishmeal based diets). Replacing fishmeal with plant based proteins will promote health through increased fish consumption and will alleviate environmental and economic constraints facing the aquaculture industry.