Molecular basis of brassinosteroid-promoted growth

BR最明显的作用是促进生长。细胞壁疏松、控制水分运输和控制纤维素生物合成已被认为是潜在的生理机制。BR对碳同化和分配、蛋白质合成和细胞壁合成前体的提供的影响也可能是相关机制。然而，尽管有大量已发表的研究，BR的主要作用模式仍不清楚，现有数据未揭示生理过程与相对充分研究的基因组事件之间的因果关系。本研究拟通过代谢组学、转录组学、形态学和超微结构分析，综合分析BR促进拟南芥叶片生长的基础。时程实验的统计分析，和植物与不同的内源BR水平的比较将允许建立剂量-反应关系和分离的主要和次要BR效应。这些实验有可能确定介导植物生长的过程。

The most obvious role of BR is growth promotion. Cell wall loosening, control of water transport, and control of cellulose biosynthesis have been suggested as underlying physiological mechanisms. BR effects on carbon assimilation and allocation, protein synthesis, and provision of precursors for cell wall synthesis may also represent involved mechanisms. However, in spite of a large number of published studies the primary mode of action of BR is still unclear, and the available data do not disclose causal relationships between physiological processes and the relatively well-studied genomic events. The proposal at hand intends an integrated analysis of the bases of BR-promoted growth in Arabidopsis leaves by means of metabolome, transcriptome, morphology, and ultrastructure analyses. Statistical analyses of time course experiments, and comparisons of plants with different endogenous BR levels will allow establishing dose-response relationships and separating primary and secondary BR effects. The experiments have the potential to identify the processes that mediate plant growth.