Optimization of Bioreactor Based Tissue Engineering of Bone

该项目采用多学科方法开发用于骨组织工程的旋转生物反应器中的新型聚合物基基质。这类基质的发展与新的组织培养技术相结合，为研究聚合物-细胞相互作用、聚合物基质对细胞反应的影响以及基质系统中运输对细胞反应的影响提供了机会。第一个项目的目标是探索开发新的聚合物结构，通过烧结微球来形成适合旋转生物反应器中骨组织工程的基质。第二个目标是优化旋转生物反应器中的支架和操作环境，优化支架的组成、支架的孔径、旋转生物反应器的转速和这些新型微球基支架周围的细胞播种密度等理化参数。第三个目标，生长因子的输送，将在生物反应器中进行研究，建立在先前的工作基础上，通过将因子纳入基质中，可以增强细胞内皮化和骨骼生长。并且，在第四个目标中，将进行广泛的体内研究，检查这些组织工程生物反应器培养基质治愈临界尺寸骨缺陷的能力。这些研究将仔细关注愈合缺陷的机械强度以及缺陷部位的组织学和组织形态学。这些研究将产生重要的新的基础信息，广泛应用于组织工程。在教育方面，这项研究预计将对本科生和研究生阶段的少数民族学生的培训产生特殊影响，从而极大地丰富我国可用的科学资源水平。Gilbert B. devey2005年4月7日

Laurencin05503207The project employs a multidisciplinary approach towards the development of novel polymer based matrices in rotating bioreactors for bone tissue engineering. The development of a matrix of this sort combined with novel tissue culture technology provides opportunities for studying polymer-cell interactions, polymer matrix effects on cellular response, and effects of transport on cellular response in matrix based systems. The first project aim is to explore the development of new polymer constructs fabricated by sintering microspheres to form matrices suitable for bone tissue engineering in rotating bioreactors. In the second aim, the scaffolds and operating environment is to be optimized in rotating bioreactors as well as the optimizing of physicochemical parameters such as scaffold composition, pore size of scaffolds, rotating speed of rotating bioreactors and cell seeding density surrounding these novel microsphere- based scaffolds. The third aim, the delivery of growth factors, will be studied in the bioreactors, building on the previous work via incorporating factors into the matrix that may enhance cellular endothelialization and bone growth. And, in the fourth aim, extensive in vivo studies will be performed, examining the ability of these tissue engineered bioreactor cultured matrices to heal critical size bone defects. These studies will pay careful attention to the mechanical strength of the healing defect and the histology and histomorphometry at the defect site. The studies should yield important new fundamental information broadly applicable in tissue engineering. Educationally, the research is expected to have a special impact on the training of underrepresented minority students at the undergraduate and graduate levels, and hence would greatly enrich the level of scientific resources available to our nation.Gilbert B. DeveyApril 7, 2005