Optimization of Bioreactor Based Tissue Engineering of Bone

基于生物反应器的骨组织工程优化

• 批准号: 0503207
• 负责人: Cato Laurencin
• 金额: --
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0503207&HistoricalAwards=false
• 关键词: Optimization; Bioreactor; Based; Tissue; Engineering

Laurencin05503207The project employs a multidisciplinary approach towards the development of novel polymer based matrices in rotating bioreactors for bone tissue engineering. The development of a matrix of this sort combined with novel tissue culture technology provides opportunities for studying polymer-cell interactions, polymer matrix effects on cellular response, and effects of transport on cellular response in matrix based systems. The first project aim is to explore the development of new polymer constructs fabricated by sintering microspheres to form matrices suitable for bone tissue engineering in rotating bioreactors. In the second aim, the scaffolds and operating environment is to be optimized in rotating bioreactors as well as the optimizing of physicochemical parameters such as scaffold composition, pore size of scaffolds, rotating speed of rotating bioreactors and cell seeding density surrounding these novel microsphere- based scaffolds. The third aim, the delivery of growth factors, will be studied in the bioreactors, building on the previous work via incorporating factors into the matrix that may enhance cellular endothelialization and bone growth. And, in the fourth aim, extensive in vivo studies will be performed, examining the ability of these tissue engineered bioreactor cultured matrices to heal critical size bone defects. These studies will pay careful attention to the mechanical strength of the healing defect and the histology and histomorphometry at the defect site. The studies should yield important new fundamental information broadly applicable in tissue engineering. Educationally, the research is expected to have a special impact on the training of underrepresented minority students at the undergraduate and graduate levels, and hence would greatly enrich the level of scientific resources available to our nation.Gilbert B. DeveyApril 7, 2005

Laurencin 05503207该项目采用多学科方法，在旋转生物反应器中开发用于骨组织工程的新型聚合物基质。这种基质与新型组织培养技术相结合的发展为研究聚合物-细胞相互作用、聚合物基质对细胞反应的影响以及基于基质的系统中运输对细胞反应的影响提供了机会。 第一个项目的目的是探索新的聚合物结构的发展，通过烧结微球，形成适合骨组织工程在旋转生物反应器的矩阵。 在第二个目标中，在旋转生物反应器中优化支架和操作环境，以及优化物理化学参数，例如支架组成、支架孔径、旋转生物反应器的转速和围绕这些新型微球基支架的细胞接种密度。第三个目标，生长因子的传递，将在生物反应器中进行研究，建立在以前的工作基础上，通过将因子掺入基质中，可以增强细胞内皮化和骨生长。并且，在第四个目标中，将进行广泛的体内研究，检查这些组织工程生物反应器培养基质愈合临界尺寸骨缺损的能力。这些研究将密切关注愈合缺损的机械强度以及缺损部位的组织学和组织形态计量学。这些研究将产生重要的新的基本信息，广泛适用于组织工程。 在教育方面，预计这项研究将对本科和研究生阶段代表性不足的少数民族学生的培训产生特殊影响，因此将大大丰富我们国家可用的科学资源水平。Devey 2005年4月7日