Bioerodible Matrices for Bone Tissue Engineering

用于骨组织工程的生物可蚀基质

• 批准号: 0336736
• 负责人: Cato Laurencin
• 金额: $ 39.6万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-15 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0336736&HistoricalAwards=false
• 关键词: Bioerodible; Matrices; Bone; Tissue; Engineering

0201923LaurencinThis research effort is aimed at developing useful biomimetic constructs for bone while furthering our fundamental understanding of factors important in determining the success of these designs. A multidisciplinary tissue engineering approach is to be followed using principles from chemical, mechanical, and materials engineering as well as cell and molecular biology. The development of constructs of this sort is expected to have clinical applications in grafting of non-unions, surgical arthrodeses, cranio-facial defects, and prosthetic implants and/or implant coatings.This research project builds on the knowledge produced during a series of research projects directed by the Principal Investigator dating from 1990. This new project seeks to design, develop and optimize three-dimensional matrices for use in bone repair. The first aim is to explore the development of new polymer-ceramic constructs fabricated by direct synthesis and incorporation of amorphous calcium phosphate into microspheres used to form the matrix, as well as optimize physicochemical parameters surrounding these new composite microspheres. In the second aim, the delivery of growth factors will also be studied further, building on the previous work by incorporating proteins and factors into the matrix that may enhance cellular endothelialization and bone growth. Finally, in the third aim, extensive in vivo studies will be performed utilizing the constructs designed from aims 1 and 2, paying careful attention to the mechanical strength of the healing defect and the short- and long-term histology and histomorphometry of the defect site.From a basic science standpoint, studies under this work have provided and will continue to provide a better understanding of polymer and ceramic chemistry as it relates to cellular response, matrix fabrication, and ultimate matrix performance (both in vitro and in vivo). In addition to furthering our basic science knowledge, the development of matrices for bone tissue engineering may lead to graft materials for healing bony defects, and thus provide direct benefits to society. As one of only a handful of underrepresented minority principal investigators in bioengineering in America, the Principal Investigator is committed to encouraging the academic growth of younger minority undergraduate and graduate students currently so underrepresented in science.

[201923] laurencin这项研究的目的是开发有用的骨仿生结构，同时进一步了解决定这些设计成功的重要因素。一个多学科的组织工程方法是遵循从化学，机械，材料工程以及细胞和分子生物学原理。这种结构体的发展有望在临床应用于骨不连、手术关节、颅面缺损、假体植入物和/或植入物涂层的移植。该研究项目建立在自1990年以来由首席研究员指导的一系列研究项目中产生的知识基础上。这个新项目旨在设计、开发和优化用于骨修复的三维基质。第一个目标是探索通过直接合成和将无定形磷酸钙掺入用于形成基体的微球中制备新型聚合物陶瓷结构的发展，以及优化这些新复合微球的物理化学参数。在第二个目标中，生长因子的传递也将进一步研究，在先前的工作基础上，通过将蛋白质和因子结合到可能增强细胞内皮化和骨骼生长的基质中。最后，在第三个目标中，将利用目标1和目标2设计的结构进行广泛的体内研究，仔细关注愈合缺陷的机械强度以及缺陷部位的短期和长期组织学和组织形态学。从基础科学的角度来看，这项工作的研究已经并将继续提供对聚合物和陶瓷化学的更好理解，因为它与细胞反应、基质制造和最终基质性能（体外和体内）有关。除了进一步加深我们的基础科学知识，骨组织工程基质的发展可能会导致骨缺损愈合的移植材料，从而为社会提供直接的利益。作为美国生物工程领域为数不多的少数族裔首席研究员之一，首席研究员致力于鼓励目前在科学领域代表性不足的年轻少数族裔本科生和研究生的学术发展。