Bioelectricity and Cell Signaling in a Microstructured Hybrid Model of Cardiac Tissue

PI将建立一个混合细胞培养模型，将心肌细胞和成纤维细胞偶联。细胞培养的拓扑结构由成纤维细胞和心脏细胞的交替条纹组成。杂交细胞培养是通过调节三嵌段共聚物的浓度来控制细胞的差异粘附。细胞网络生长在一个灵活的微槽上，由于它的灵活性，对目标细胞施加机械应力。PI显示的初步结果表明，当电刺激时，细胞网络释放钠肽（NP）（应激释放的一种化学物质）。NP的升高可以通过钙阻滞剂消除，这有力地支持了NP释放是由机械应力诱导的事实。

0503336EntchevaABSTRACTThe PI will build a hybrid cell culture model, which couples cardiac myocytes and fibroblasts. The topology of the cell culture consists of alternate stripes of fibroblasts and cardiac cells. The hybrid cell culture is built by differential cell adhesion, which is controlled by adjusting the concentration of a triblock copolymer. The cell network is grown on a flexible microgroove, which, due to its flexibility, exerts mechanical stress on targeted cells. The PI shows preliminary results indicating that the cell network, when electrically stimulated, releases natriuretic peptides (NP) (one of the chemical release by stress). The elevation of NP can be eliminated with a calcium blocker, strongly supporting the fact that NP releases is induced by mechanical stress.