Bioelectricity and Cell Signaling in a Microstructured Hybrid Model of Cardiac Tissue

心脏组织微结构混合模型中的生物电和细胞信号传导

• 批准号: 0503336
• 负责人: Emilia Entcheva
• 金额: $ 29.93万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0503336&HistoricalAwards=false
• 关键词: Bioelectricity; Cell; Signaling; Microstructured; Hybrid

0503336EntchevaABSTRACTThe PI will build a hybrid cell culture model, which couples cardiac myocytes and fibroblasts. The topology of the cell culture consists of alternate stripes of fibroblasts and cardiac cells. The hybrid cell culture is built by differential cell adhesion, which is controlled by adjusting the concentration of a triblock copolymer. The cell network is grown on a flexible microgroove, which, due to its flexibility, exerts mechanical stress on targeted cells. The PI shows preliminary results indicating that the cell network, when electrically stimulated, releases natriuretic peptides (NP) (one of the chemical release by stress). The elevation of NP can be eliminated with a calcium blocker, strongly supporting the fact that NP releases is induced by mechanical stress.

0503336 Entcheva摘要主要研究者将建立一个杂交细胞培养模型，该模型将心肌细胞和成纤维细胞偶联。细胞培养物的拓扑结构由成纤维细胞和心肌细胞的交替条纹组成。通过调节三嵌段共聚物的浓度来控制差异细胞粘附，从而建立杂交细胞培养物。细胞网络在柔性微槽上生长，由于其柔性，其对靶细胞施加机械应力。PI显示了初步结果，表明当电刺激时，细胞网络释放利钠肽（NP）（应激释放的化学物质之一）。NP的升高可以用钙阻滞剂消除，强烈支持NP释放是由机械应力诱导的事实。