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UNS: All-Optical Interrogation System for Cardiac Dynamics

UNS：心脏动力学全光学询问系统

基本信息

批准号：
1623068
负责人：
Emilia Entcheva
金额：
$ 29.43万
依托单位：
George Washington University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2015
资助国家：
美国
起止时间：
2015-12-31 至 2019-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1623068&HistoricalAwards=false
关键词：
UNS Optical Interrogation System Cardiac

项目摘要

PI: Entcheva, EmiliaProposal: 1511353The PI will develop and validate a new dynamic space-time resolved all-optical approach to cardiac dynamics. The unique advantages of optogenetic actuation will be combined with simultaneous high-resolution optical imaging to offer a conceptually new way of quantifying cardiac instabilities and predict arrhythmias.They will demonstrate a general framework for contactless probing of biological function in space-time by optogenetic means that can be applied not only to perturbing and observing membrane potential in excitable cells, but also for the study of cell signaling, gene expression, for which optogenetic tools exist. The all-optical methodology allows for straightforward integration of real-time feedback control in vitro and in vivo, which can yield new insights into biological function and ability to control it. To broaden the educational impact of the proposed work, the PI, a dedicated mentor of early-stage trainees, will: 1) collaborate with the BioPREP program at Stony Brook to recruit academically-strong community college students from underprivileged background, helping them pursue a career in STEM; and 2) will integrate the developed techniques into a new formal course on quantitative optogenetic approaches to the study of biological functions, which will be offered not only at Stony Brook in a regular format, but also online via the Coursera educational platform. The evaluation of educational outcomes will be done by tracking of the career paths of trained students via social media.

PI：Entcheva，Emily Proposal：1511353 PI将开发和验证一种新的动态时空分辨全光学心脏动力学方法。光遗传驱动的独特优势将与同步高分辨率光学成像相结合，为量化心脏不稳定性和预测心律失常提供一种全新的概念方法。它们将展示用光遗传方法在时空中非接触探测生物功能的一般框架，不仅可以应用于扰动和观察可兴奋细胞的膜电位，还可以用于研究细胞信号和基因表达，而光遗传工具已经存在。全光学方法允许在体外和体内进行实时反馈控制的直接集成，这可以对生物功能和控制它的能力产生新的见解。为了扩大拟议工作的教育影响，作为早期受训人员的专门导师，国际学生联合会将：1)与石溪大学的BioPREP计划合作，招募来自贫困家庭的学业优秀的社区大学生，帮助他们在STEM中发展事业；2)将开发的技术整合到一门新的正式课程中，内容是研究生物功能的量化光遗传学方法，该课程不仅将以常规形式在石溪大学提供，还将通过Coursera教育平台在线提供。对教育成果的评估将通过社交媒体跟踪受过培训的学生的职业道路来完成。