Multifunctional colloidal nanoparticles for dual imaging with fluorescence, magnitic and radioactive detection

用于荧光、磁性和放射性检测双重成像的多功能胶体纳米粒子

• 批准号: 170468592
• 负责人: Professor Dr. Wolfgang Parak
• 金额: --
• 依托单位: Institut für Nanostruktur- und Festkörperphysik (INF)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/170468592?language=en
• 关键词: Multifunctional; colloidal; nanoparticles; dual; imaging

Colloidal nanoparticles composed out of an inorganic core and a polymer shell will be synthesized. Both, the core and the polymer shell will be either fluorescence, magnetic, or radioactive, so that they can be imaged with fluorescence, magnetic resonance, or radioactivity, respectively. By combining different cores with different polymer shells nine different types of particles for dual imaging will be obtained, as for example fluorescent cores with radioactive polymer shells. In this way a toolkit of nine types of nanoparticles will be created out of which each can be imaged with two different modes. Due to the topography of the polymer shell all nine different types of particles will posses the same surface chemistry and thus have the same interface for consecutive conjugation with ligands. Double labelling will allow for examining the fate of nanoparticles which are incorporated by living cells, for example to find out if the polymer shell around the core is digested or released. Demonstration experiments with double labelling will involve the full band width of particle distribution within organs (on a whole animal level), within tissue derived from the organs (on a tissue level) up to distribution within cells derived from the tissue (on a single cell level).

将合成由无机核和聚合物壳组成的胶体纳米颗粒。核和聚合物壳都将是荧光的、磁性的或放射性的，使得它们可以分别用荧光、磁共振或放射性成像。通过将不同的核与不同的聚合物壳组合，将获得用于双重成像的九种不同类型的颗粒，例如具有放射性聚合物壳的荧光核。通过这种方式，将创建九种类型的纳米颗粒的工具包，其中每种都可以用两种不同的模式成像。由于聚合物壳的形貌，所有九种不同类型的颗粒将具有相同的表面化学性质，因此具有相同的界面用于与配体连续缀合。双标记将允许检查被活细胞掺入的纳米颗粒的命运，例如，以确定核心周围的聚合物壳是否被消化或释放。采用双标记的示范实验将涉及器官内（在整个动物水平上）、源自器官的组织内（在组织水平上）直至源自组织的细胞内（在单细胞水平上）的颗粒分布的全带宽。

项目成果

Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing

• DOI: 10.1117/1.jbo.19.10.101506
• 发表时间: 2014-10-01
• 期刊: JOURNAL OF BIOMEDICAL OPTICS
• 影响因子: 3.5
• 作者: Cywinski, Piotr J.;Hammann, Tommy;Loehmannsroeben, Hans-Gerd
• 通讯作者: Loehmannsroeben, Hans-Gerd

Polymer-coated nanoparticles: a universal tool for biolabelling experiments.

• DOI: 10.1002/smll.201100608
• 发表时间: 2011-11
• 期刊: Small
• 影响因子: 13.3
• 作者: Feng Zhang;Emma E. Lees;F. Amin;Pilar Rivera Gil;Fang Yang;P. Mulvaney;W. Parak