Multifunctional colloidal nanoparticles for dual imaging with fluorescence, magnitic and radioactive detection

将合成由无机核和聚合物壳组成的胶体纳米颗粒。核心和聚合物外壳都将是荧光的、磁性的或放射性的，因此它们可以分别用荧光、磁共振或放射性成像。通过将不同的核心与不同的聚合物外壳相结合，将获得用于双重成像的9种不同类型的粒子，例如具有放射性聚合物外壳的荧光核心。通过这种方式，将创建一个包含九种纳米颗粒的工具包，其中每一种都可以用两种不同的模式进行成像。由于聚合物壳层的拓扑结构，所有九种不同类型的粒子都具有相同的表面化学，因此具有相同的界面，以便与配体连续连接。双重标记将允许检查被活细胞结合的纳米颗粒的命运，例如，找出核心周围的聚合物外壳是否被消化或释放。双标记的演示实验将涉及粒子在器官内(在整个动物水平上)、从器官来源的组织内(在组织水平上)到在组织来源的细胞内(在单细胞水平上)分布的全频带宽度。

Colloidal nanoparticles composed out of an inorganic core and a polymer shell will be synthesized. Both, the core and the polymer shell will be either fluorescence, magnetic, or radioactive, so that they can be imaged with fluorescence, magnetic resonance, or radioactivity, respectively. By combining different cores with different polymer shells nine different types of particles for dual imaging will be obtained, as for example fluorescent cores with radioactive polymer shells. In this way a toolkit of nine types of nanoparticles will be created out of which each can be imaged with two different modes. Due to the topography of the polymer shell all nine different types of particles will posses the same surface chemistry and thus have the same interface for consecutive conjugation with ligands. Double labelling will allow for examining the fate of nanoparticles which are incorporated by living cells, for example to find out if the polymer shell around the core is digested or released. Demonstration experiments with double labelling will involve the full band width of particle distribution within organs (on a whole animal level), within tissue derived from the organs (on a tissue level) up to distribution within cells derived from the tissue (on a single cell level).