NER: Exploration of a Nano-engineered Flagellar Motor Based TNT Detection System

NER：基于纳米工程鞭毛电机的 TNT 检测系统的探索

• 批准号: 0508435
• 负责人: Min Zou
• 金额: --
• 依托单位: University of Arkansas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0508435&HistoricalAwards=false
• 关键词: NER; Exploration; Nano; engineered; Flagellar

NER: Exploration of a Nano-engineered Flagellar Motor Based TNT Detection System PROJECT SUMMARY REVISED (6/17/2005) The goal of this project is to explore the possibility of a hybrid biological/artificial system to effectively detect trace level of Trinitrotoluene (TNT). The hybrid system is fabricated from MEMS devices and non-pathogenic, genetically engineered flagellar motors. The operational principle is based on detecting the rotational characteristics of the flagellar filament in the presence of TNT. The detection system is expected to have superior sensitivity, very short response time, and is compact, portable, and highly scalable. Based on preliminary calculations and studies, the system is at least 100 times lighter and would be much cheaper than commercially available TNT detection systems of similar sensitivity levels. The detection system will be fabricated based on the core flagellar motor assembly technologies developed by the PIs' research groups. These technologies will be further advanced by completing the three complementary objectives identified for the proposed project: 1) Develop methodologies for tethering cell body to substrate surfaces, 2) Develop biological protocols for specific bead attachment, 3) Integrate detection system and evaluate performance.

NER：探索基于纳米工程鞭毛马达的三硝基甲苯检测系统项目摘要修订(2005年6月17日)该项目的目标是探索一种生物/人工混合系统的可能性，以有效地检测痕量三硝基甲苯(TNT)。该混合系统是由MEMS器件和非致病的、基因工程的鞭毛马达制造的。其工作原理是基于在TNT存在下检测鞭毛细丝的旋转特性。预计该检测系统将具有优越的灵敏度、非常短的响应时间、紧凑、便携和高度可扩展的特点。根据初步计算和研究，该系统至少轻100倍，比市场上类似灵敏度水平的TNT探测系统便宜得多。探测系统将基于PIS研究小组开发的核心鞭毛马达组装技术制造。这些技术将通过完成为拟议项目确定的三个互补目标而进一步推进：1)开发将细胞体与底物表面连接的方法，2)开发特定珠子附着的生物协议，3)集成检测系统和评估性能。