Induction of plant metabolites and feed-back on herbivores

植物的代谢模式是其生理状态的反映，受多种内外部因素的影响。本文将重点研究拟南芥对地上和地下取食食草动物的代谢物谱变化的时空格局以及植物变化对这些生物的反馈效应，以揭示生态相互作用的机制。研究的目的是：(1)筛选地上（蚜虫）或地下（寄生线虫）取食食草物种所诱发的茎和根组织代谢物诱导模式和时间过程；(2)确定物种特异性诱导的关键代谢物；(3)研究代谢谱变化对食草动物行为和性能的反馈机制，从而研究植物性状介导的蚜虫和线虫之间的间接相互作用。从长远来看，数据应该与植物基因表达谱联系起来，以评估介导这种串扰生理过程的信号通路。预计不同摄食群体的食草动物会导致局部和系统的高度特异性植物反应，而食草动物也会在不同程度上受到代谢物变化的影响。

The metabolite pattern of a plant is a mirror of its physiological status and depends on diverse internal and external factors. The spatial and temporal pattern of changes in metabolite profiles of the model plant Arabidopsis thaliana in response to above- and belowground feeding herbivores and feedback effects of plant changes on these organisms will be in focus of this study to reveal mechanisms of ecological interactions. The aims are (I) to screen the pattern and time-course of metabolite induction in shoot and root tissue evoked by either aboveground (aphids) or belowground (endoparasitic nematodes) feeding herbivore species, (II) to identify key metabolites that are species-specifically induced and (III) to investigate the feed-back mechanisms of changed metabolic profiles on the herbivore behaviour and performance and thus the plant trait-mediated indirect interactions between aphids and nematodes. In the long term, data should be linked to plant gene-expression profiles to evaluate the signalling pathways mediating the physiological processes of this cross-talk. It is expected that herbivores of different feeding groups lead to locally and systemically highly specific plant responses and that the herbivores in turn are also affected to varying degrees by metabolite changes.