Chip-Scale Integrated Nanofluidics/Affinity Microcolumn Biosensors

芯片级集成纳米流体/亲和微柱生物传感器

• 批准号: 0515684
• 负责人: Steven R. J. Brueck
• 金额: --
• 依托单位: University of New Mexico
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-15 至 2010-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0515684&HistoricalAwards=false
• 关键词: Chip; Scale; Integrated; Nanofluidics; Affinity

ABSTRACTNSF-0515684Brueck, StevenDetection of chemical and biological warfare (CBW) agents is a con-tinuing challenge. The amounts of CBW agent sufficient to cause harm to humans are very small, requiring exceptional sensitivity; often, rapid identification and remediation is necessary; widely dispersed, inexpensive sensors are required to monitor large areas for agents; and, perhaps the most worrisome, the variety of possible agents is virtually unbounded. With advances in biological synthesis capabilities, creation of new CBW agents is no longer exclusively a nation-state enterprise.This development demands a new approach to monitoring. In order to meet the sensitivity and areal distribution requirements, a chip-scale technology, sensitive to a variety of agent classes, requiring only very small (picoliter) volumes and with integral preconcentration processes is required. The broad goal of this work is to establish the basic understanding andthe technological methodologies necessary to implement high-density arrays of nanoscale channels as versatile, sensitive and selective components for chip-scale biodetection systems that will allow response to a wide variety of CBW agents. Nanofluidic separation and detection (providing the picoliter sensitivity) and smart, functionalized receptor bead microcolumns (providing the preconcentration and selectivity). Technologies are brought together. A fundamental understanding of the physical phenomena involved in nanoscale transport of complex fluids is a prerequisite for the development of the new generation of bio-separationtechnologies. A key enabling technology that will emerge from this effort is the development of inexpensive, facile and manufacturable means for creating integrated fluidic circuits that allow the transition from macroscopic fluid handling (e.g., pipettes) to nanoscale dimensions.This program will support two students and one postdoctoral re-searcher. UNM is a Hispanic Serving Institution with large populations of Hispanic and Native American students;very active recruitment and retention efforts are already underway in the labs of the investigators.

化学和生物战（CBW）毒剂的检测是一个持续的挑战。足以对人类造成伤害的生化武器制剂的量非常小，需要特别的灵敏度；通常，快速识别和补救是必要的；需要广泛分散、价格低廉的传感器来监测大面积的病原体；而且，也许最令人担忧的是，可能的代理人的种类几乎是无限的。随着生物合成能力的进步，新的生化武器制剂的创造不再仅仅是一个国家的企业。这种发展需要一种新的监测方法。为了满足灵敏度和面积分布要求，需要一种芯片级技术，对各种类型的药剂敏感，只需要非常小的（皮升）体积，并具有完整的预浓缩过程。这项工作的总体目标是建立基本的理解和必要的技术方法，以实现纳米级通道的高密度阵列，作为芯片级生物检测系统的通用、敏感和选择性组件，这将允许对各种CBW试剂做出反应。纳米流体分离和检测（提供皮升灵敏度）和智能，功能化受体珠微柱（提供预浓缩和选择性）。技术融合在一起。对复杂流体纳米级传输中涉及的物理现象的基本理解是开发新一代生物分离技术的先决条件。这项工作将产生一项关键的使能技术，即开发廉价、简便和可制造的方法，用于创建集成流体电路，使其能够从宏观流体处理（例如移液器）过渡到纳米尺度。本项目资助学生2名，博士后1名。新墨西哥大学是一所西班牙裔服务机构，拥有大量西班牙裔和美洲原住民学生；在调查人员的实验室里，非常积极的招聘和保留工作已经在进行中。