Mechanistic Studies of Biomolecular Ion Fragmentation Induced by Vacuum Ultraviolet Laser Light

真空紫外激光诱导生物分子离子断裂的机理研究

• 批准号: 0518234
• 负责人: James Reilly
• 金额: $ 37.3万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0518234&HistoricalAwards=false
• 关键词: Mechanistic; Studies; Biomolecular; Ion; Fragmentation

Professor James P. Reilly of Indiana University is supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to conduct mechanistic studies of biomolecular ion fragmentation induced by vacuum ultraviolet laser light. Deuterium isotope experiments will be used to probe the mechanisms of fragmentation of peptides and proteins. The dependence of the fragmentation profiles on laser light wavelength, and the effect of variations in incident laser light intensity on the observed fragmentation profiles will also be examined. Further, the effects of protein phosphorylation and glycosylation on the ability of this technique to determine protein sequence information will be studied.The research promises to provide basic understanding of the fundamentals of the vacuum ultraviolet laser ligh photoionization of peptides, and to develop mass spectrometric tools and methods for proteomic analysis. The project has the potential for high impact in mass spectrometry as well as in bioanalytical chemistry where researchers need improved tools for analysis of proteins.

印第安纳大学的James P. Reilly教授在化学系分析和表面化学项目的支持下，对真空紫外激光诱导的生物分子离子碎裂进行了机制研究。氘同位素实验将用于探索多肽和蛋白质断裂的机制。研究了激光波长对碎片分布的影响，以及入射激光强度的变化对碎片分布的影响。此外，蛋白质磷酸化和糖基化对该技术确定蛋白质序列信息能力的影响将被研究。该研究有望提供对真空紫外激光光电离肽的基本原理的基本理解，并开发用于蛋白质组学分析的质谱工具和方法。该项目在质谱分析以及生物分析化学方面具有很大的影响潜力，因为研究人员需要改进蛋白质分析工具。