Acquisition of a high-Resolution Data Collection System

获取高分辨率数据收集系统

• 批准号: 0521553
• 负责人: James Sacchettini
• 金额: $ 68.42万
• 依托单位: Texas A&M AgriLife Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0521553&HistoricalAwards=false
• 关键词: Acquisition; Resolution; Data; Collection; System

The X-ray crystallography data collection station funded by this proposal represents the first of a new generation of very high intensity X-ray generators that, when coupled with VariMax High-Resolution and High-Flux optics and a high resolution image plate detector, can give near synchrotron results in a home source. The system consists of a MicroMax007 High-Flux x-ray generator with VariMax high-resolution optics capable of generating a beam flux of 2.95x10^9photons/sec, almost equal to a 3rd generation synchrotron bending magnet line. The data collection station will greatly increase the capabilities of several structural biology research programs at Texas A&M by allowing scientists rapid access to these facilities. The six areas of structural biology most impacted by the instrument will be: high-resolution structure determination, rapid inhibitor screening, membrane structure determination, protein folding, enzyme mechanism, and protein-protein interactions. This instrumentation will also aid in the development of software packages for automated structure determination and in-silco screening. The multidisciplinary group of faculty and students within the Departments of Biology, Biochemistry and Biophysics, Chemistry, and Computer Science, comprise the Texas A&M Center for Structural Biology and will be the primary users of the instrument. These research programs interface their projects to bridge gaps between other methods in structural sciences including NMR, fluorescence, CD, computational biology, and protein crystallography, ultimately providing a seamless approach to understanding the details of protein structure and function. The X-ray data collection equipment requested in this proposal will allow for a community of researchers to expand their research into structural biology. This will aid in our efforts to create new collaborations, while enhancing existing collaborations. Cross training efforts among the fields of biology, biochemistry, immunology and biophysics will be greatly increased, aiding in the education of both graduate and post-doctoral students. In doing so, the improved training will ultimately increase the recruitment power, making Texas A&M University more inviting to underrepresented scientists. Undergraduate and graduate education, with hands on experience, will be improved as this instrumentation is incorporated into the structural biology curriculum.

由该提案资助的x射线晶体学数据收集站代表了新一代非常高强度x射线发生器的第一个，当与VariMax高分辨率和高通量光学器件以及高分辨率图像板探测器相结合时，可以在家庭源中获得接近同步加速器的结果。该系统由MicroMax007高通量x射线发生器组成，具有VariMax高分辨率光学器件，能够产生2.95x10^9光子/秒的光束通量，几乎相当于第三代同步加速器弯曲磁力线。该数据收集站将允许科学家快速访问这些设施，从而大大提高德克萨斯农工大学几个结构生物学研究项目的能力。受该仪器影响最大的六个结构生物学领域将是：高分辨率结构测定、快速抑制剂筛选、膜结构测定、蛋白质折叠、酶机制和蛋白质-蛋白质相互作用。该仪器还将有助于开发用于自动化结构确定和硅内筛选的软件包。来自生物系、生物化学和生物物理系、化学系和计算机科学系的多学科师生组成了德克萨斯农工大学结构生物学中心，他们将是该仪器的主要用户。这些研究项目将他们的项目连接起来，以弥合结构科学中其他方法之间的差距，包括核磁共振、荧光、CD、计算生物学和蛋白质晶体学，最终提供了一种无缝的方法来理解蛋白质结构和功能的细节。本提案中要求的x射线数据收集设备将允许研究人员将他们的研究扩展到结构生物学。这将有助于我们努力创造新的合作，同时加强现有的合作。将大力加强生物学、生物化学、免疫学和生物物理学领域的交叉训练，帮助培养研究生和博士后。这样一来，改进的培训将最终增加招聘能力，使德克萨斯农工大学更能吸引那些代表性不足的科学家。随着该仪器被纳入结构生物学课程，具有实践经验的本科和研究生教育将得到改善。