Biophotonics: Membranes as Motors: Investigating Nanoelectromechanical Characteristics

申请人的主要目标是了解在细胞膜中电诱导皮牛子水平的力产生的机制(S)，以及通过膜的电/机械性质以及跨膜蛋白(如Prestin)和阴离子两亲性试剂(如水杨酸盐)的相互作用来调节这一过程的分子基础。具体目标包括：(1)研究在同时缺乏prestin和细胞骨架的情况下，膜表面电荷和张力对电诱导力生成的影响；(2)使用模型野生型人胚胎肾(HEK)细胞和转染膜前蛋白(A)、(B)突变的prestin以及(C)与prestin密切相关的其他蛋白质的HEK细胞，研究prestin在整体电诱导力生成中的作用；以及(3)使用在膜上掺入电压敏感荧光染料来研究prestin与机电的相互作用。

0522862AnvariABSTRACTThe applicant's main objectives are to understand the mechanism(s) of the electrically-induced piconewton level force generation in cell membranes and the molecular basis for modulation of this process through membrane electrical/mechanical properties and the interaction of transmembrane proteins such as prestin and anionic amphipathic agents such as solicyate. Specific objectives include: (1) Investigate the effects of membrane surface charge and tension on electrically-induced force generation in the absence of both prestin and the cytoskeleton; (2) Investigate the contribution of prestin to the overall electrically-induced force generation using model wildtype human embryonic kidney (HEK) cells, and HEK cells transfected with: (a) prestin, (b) mutated prestin, and (c) other proteins closely-related to prestin; and (3) Investigate electromechanical reciprocity using a voltage sensitive fluorescent dye incorporated into the membrane.