Biophotonics: Membranes as Motors: Investigating Nanoelectromechanical Characteristics

本申请人的主要目的是了解细胞膜中电诱导皮牛顿水平力产生的机制，以及通过膜电/机械性质和跨膜蛋白如普雷斯廷和阴离子两亲性试剂如sociyate的相互作用来调节该过程的分子基础。具体目标包括：（1）研究在不存在普雷斯廷和细胞骨架的情况下膜表面电荷和张力对电诱导力产生的影响;（2）使用模型野生型人胚肾（HEK）细胞和用以下转染的HEK细胞研究普雷斯廷对总电诱导力产生的贡献：（a）普雷斯廷，（B）突变的普雷斯廷，和（c）与普雷斯廷密切相关的其它蛋白质;和（3）使用掺入膜中的电压敏感荧光染料研究机电相互作用。

0522862AnvariABSTRACTThe applicant's main objectives are to understand the mechanism(s) of the electrically-induced piconewton level force generation in cell membranes and the molecular basis for modulation of this process through membrane electrical/mechanical properties and the interaction of transmembrane proteins such as prestin and anionic amphipathic agents such as solicyate. Specific objectives include: (1) Investigate the effects of membrane surface charge and tension on electrically-induced force generation in the absence of both prestin and the cytoskeleton; (2) Investigate the contribution of prestin to the overall electrically-induced force generation using model wildtype human embryonic kidney (HEK) cells, and HEK cells transfected with: (a) prestin, (b) mutated prestin, and (c) other proteins closely-related to prestin; and (3) Investigate electromechanical reciprocity using a voltage sensitive fluorescent dye incorporated into the membrane.