SST: Collaborative Research: Integrated Optical and Electrical Single Molecule Sensors

0528730施密特光学和电学传感使研究气体或液体中的单个分子成为可能，从而在非常基本的水平上理解物理、化学和生物过程。提出了一种集成传感器平台，它在传感器阵列配置中提供单分子分辨率、极小的气体或液体样品体积、平面结构和高通量。这种传感器非常适合于提供快速、可靠和坚固的分析工具。这些传感器不需要庞大的三维设置，也可以在实验室外使用，可以影响从基因组和蛋白质组到毒理学和空气和水中污染监测的各种领域。智能优势：建议的方法基于纳米孔技术与单分子电分辨率和带有液体芯的反共振反射光(ARROW)光波导的结合。本研究的主要目标有以下几个方面：1.构建一种新型的集成光学传感器，能够同时提供单分子荧光灵敏度，并允许并行的传感器阵列结构。2.将纳米孔作为“智能门”与光波导集成。纳米孔将控制分子在光学检测通道中的访问，同时提供单分子分辨率的电传感能力。利用光电传感的新组合来研究基因组学和蛋白质组学中的生物分子，以加强在单分子水平上对分子生物学的理解。虽然这个概念原则上同样适用于气相分子的传感，但这项提议的重点是用于单分子水平生物分子研究的集成液体传感器。更广泛的影响：上面概述的研究计划具有重大的更广泛的影响。它将促进光学传感器科学和技术的发展，并在集成光学、分子生物学和半导体器件制造领域提供研究生培训。本科生研究将被整合到该项目中。在加州大学洛杉矶分校，学生将在现有的加州大学领导班子(代表不足的少数族裔)或COSMOS(K-12学生)计划下参与该项目。在杨百翰大学，来自代表不足群体(杨百翰SOAR项目)的本科生和高中生将在夏季参与项目的制作方面。

0528730SchmidtOptical and electrical sensing has enabled the study of individual molecules in the gas or liquid phase, leading to an understanding of physical, chemical and biological processes on a very fundamental level. An integrated sensor platform is proposed that provides single-molecule resolution, extremely small gas or liquid sample volumes, planar architecture, and high throughput in a sensor array configuration. Such a sensor is highly desirable for providing fast, reliable and robust analytical tools. Without the need for bulky three-dimensional setup, these sensors can also be used outside a laboratory and could impact a variety of fields ranging from genomics and proteomics to toxicology and pollution monitoring in both air and water.Intellectual merit: The proposed approach is based on combining nanopore technology with single-molecule electrical resolution and antiresonant reflecting optical (ARROW) waveguides with liquid cores. The research has the following primary goals: 1. To build a new type of integrated optical sensor that simultaneously provides single-molecule fluorescence sensitivity and allows for parallel sensor array architectures. 2. To integrate nanopores as "smart gates" with the optical waveguides. The nanopores will control molecule access in the optical detection channel and simultaneously provide electrical sensing capability with single molecule resolution.3. To use the novel combination of optical and electrical sensing to study biomolecules in genomics and proteomics for enhancing understanding of molecular biology on the single molecule level. While the concept is, in principle, equally applicable to sensing of molecules in the gas phase, the focus of this proposal is on integrated sensors of liquids for biomolecular studies on the single molecule level.Broader Impact: The research program outlined above has significant broader impact. It will advance optical sensor science and technology and provide graduate student training in the fields of integrated optics, molecular biology, and semiconductor device fabrication. Undergraduate research will be integrated into the program. At UCSC, students will participate in the project under the existing UC LEADS (underrepresented minorities) or COSMOS (K-12 students) programs. At BYU, undergraduates as well as high school students from underrepresented groups (BYU SOAR program) will be involved in the fabrication aspects of the projects during the summer.