Biophotonic devices for sample-to-answer biomarker analysis

用于样本到答案生物标志物分析的生物光子设备

• 批准号: 1703058
• 负责人: Holger Schmidt
• 金额: $ 43.22万
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1703058&HistoricalAwards=false
• 关键词: Biophotonic; devices; sample; answer; biomarker

This proposal will devise an optofluidic versatile system that will allow pre-analysis manipulation of the sample for removing components that might interfere with the analysis. Using nucleic acids of the target analyte along and with antibodies to detect the presence of a variety of infection-causing viruses in the presence other viruses. The device will be engineered on chips with little a-priori information on the content of the target sample. This is the sample-to-answer system where the developed chip would be available for a variety of yet underdetermined diagnostic tasks. This goal is a quantum leap from the current target-dedicated diagnostics on a chip to the all-purpose diagnostic chip with as broad an application as possible without sacrificing capabilities of sensitivity, selectivity, ease of use and cost. The specific objectives are to develop a multi-wavelength waveguide that will provide a multi-source for excitation of biomarkers. A second objective is to develop a sample preparation section on the chip. The final effort in this work will demonstrate the high sensitivity and selectivity of the approach by detecting the Zika virus using both, nucleic acid and biomarkers for the virus in the presence other viruses. The present proposal evolves from a long standing collaboration between the present PI, a physicist in Electrical Engineering at UC Santa Cruz, and a the Hawkins group at BYU. The previous achievements of the collaboration are detailed in the proposal and has enjoyed broad recognition within the community and beyond.

该提案将设计一种光流体通用系统，该系统将允许对样品进行分析前操作，以去除可能干扰分析的组分。使用目标分析物的核酸沿着并与抗体一起检测在存在多种引起感染的病毒的情况下是否存在其它病毒。该设备将在芯片上设计，几乎没有关于目标样品内容的先验信息。这是一个样本到答案的系统，开发的芯片将可用于各种尚未确定的诊断任务。这一目标是从目前的芯片上的目标专用诊断到具有尽可能广泛的应用的通用诊断芯片的一个巨大飞跃，而不会牺牲灵敏度，选择性，易用性和成本。具体目标是开发一种多波长波导，其将提供用于激发生物标志物的多源。第二个目标是在芯片上开发样品制备部分。这项工作的最后努力将通过在存在其他病毒的情况下使用病毒的核酸和生物标志物检测寨卡病毒来证明该方法的高灵敏度和选择性。目前的建议是从目前的PI，在加州大学圣克鲁斯电气工程物理学家，和杨百翰大学霍金斯组之间的长期合作发展而来的。合作的先前成就在建议书中有详细说明，并在社区内外得到广泛认可。

项目成果

Optofluidic Distributed Feedback Dye Laser Using Corrugated Sidewall Structure

采用波纹侧壁结构的光流控分布式反馈染料激光器

• 发表时间: 2019
• 期刊: PVLED
• 作者: Black, JA.

Optofluidic detection of Zika nucleic acid and protein biomarkers using multimode interference multiplexing

• DOI: 10.1364/boe.9.003725
• 发表时间: 2018-08-01
• 期刊: BIOMEDICAL OPTICS EXPRESS
• 影响因子: 3.4
• 作者: Stambaugh, Alexandra;Parks, Joshua W.;Schmidt, Holger
• 通讯作者: Schmidt, Holger

Enhanced Detection of Single Viruses On-Chip via Hydrodynamic Focusing

• DOI: 10.1109/jstqe.2018.2854574
• 发表时间: 2019-01-01
• 期刊: IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
• 影响因子: 4.9
• 作者: Black, Jennifer A.;Hamilton, Erik;Schmidt, Holger
• 通讯作者: Schmidt, Holger

Single Particle Detection Enhancement with Wavelet-based Signal Processing Technique

利用基于小波的信号处理技术增强单粒子检测

• DOI: 10.1364/cleo_si.2019.stu3h.4
• 发表时间: 2019
• 作者: Ganjalizadeh, V.;Meena, G.G.;Stott, M.A.;Schmidt, H.;Hawkins, A.R.
• 通讯作者: Hawkins, A.R.

3D Hydrodynamic Focusing in Microscale Optofluidic Channels Formed with a Single Sacrificial Layer

• DOI: 10.3390/mi11040349
• 发表时间: 2020-04-01
• 期刊: MICROMACHINES
• 影响因子: 3.4
• 作者: Hamilton, Erik S.;Ganjalizadeh, Vahid;Hawkins, Aaron R.
• 通讯作者: Hawkins, Aaron R.