Assembing the Tree of Life: An Integrative Approach to Investigating Cnidarian Phylogeny

组装生命之树：研究刺胞动物系统发育的综合方法

• 批准号: 0531763
• 负责人: Marymegan Daly
• 金额: $ 56.51万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-10-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0531763&HistoricalAwards=false
• 关键词: Assembing; Tree; Life; Integrative; Approach

The phylum Cnidaria is one of the earliest evolving animal groups and includes prominent members of the marine fauna such as corals, sea anemones and jellyfish. Because cnidarians diverged from the rest of the animals prior to the evolution of organs, their overall anatomy is quite simple. Despite this however, cnidarians have attained incredible diversity, comprising over 11,000 species, many of which display elaborate structures and complex life histories. The lack of organs and other anatomical landmarks has made it difficult for scientists to discern their evolutionary relationships. Recent technological and theoretical advances in the sequencing and analysis of DNA provide the opportunity to use the information encoded within an organism's genetic code to elucidate evolutionary relationships. The primary aim of this proposal is to obtain DNA sequence data from 1,800 cnidarian species: this will generate approximately 23 million base pairs of cnidarian DNA sequence, and the specimens from which the sequences are obtained will be retained in natural history museum collections. The information encoded in the DNA will be used to reconstruct cnidarian phylogenies (evolutionary trees), using computer algorithms and supercomputing facilities. Some of the questions this proposal will address are: Was the ancestor to all of cnidarians solitary or colonial? Are jellyfish or hydras more closely related to the corals? How and when did coral skeletons evolve? What are the evolutionary transitions between a simple hydra polyp and a complex Portuguese Man-of-War colony? Reconstructing a cnidarian evolutionary tree is important for our understanding of the patterns and processes that accompanied the early diversification of animal life. Formal and informal education will result from this research. Undergraduate, graduate, and postdoctoral scholars, including those belonging to groups under-represented in the sciences, will be trained. A symposium on cnidarian evolutionary relationships will be organized by the investigators at a national scientific meeting. A Cnidaria Tree of Life database will be developed to include outreach and learning components such as images, common names, and keys designed to appeal to students at all levels as well as interested users such as divers and aquarists. Museum exhibits will be created based on research results, one at the University of Kansas Natural History Museum, and one, entitled "Cnidarian Diversity in the Oceans" that will be part of the new Oceans Hall, due to open at the National Museum of Natural History in 2008. A website and classroom poster (in both English and Spanish) integrated with the latter exhibit will be created in collaboration with a K-12 educator supported through NSF's Research Experience for Teachers program.

刺胞动物门是最早进化的动物群体之一，包括珊瑚、海葵和水母等海洋动物的杰出成员。因为刺胞动物在器官进化之前就与其他动物分道扬镳，所以它们的整体解剖结构相当简单。然而，尽管如此，刺胞动物已经达到了令人难以置信的多样性，包括超过11,000种，其中许多显示出精致的结构和复杂的生活史。由于缺乏器官和其他解剖标志，科学家很难辨别它们的进化关系。最近在DNA测序和分析方面的技术和理论进步提供了利用生物体遗传密码内编码的信息来阐明进化关系的机会。本提案的主要目的是获取1800种刺胞动物的DNA序列数据：这将产生大约2300万个碱基对的刺胞动物DNA序列，从这些序列中获得的标本将被自然历史博物馆收藏。编码在DNA中的信息将用于利用计算机算法和超级计算设备重建刺胞动物的系统发育（进化树）。这一提议将解决的一些问题是：所有刺胞动物的祖先是独居的还是群居的？水母和水螅与珊瑚的关系更近吗？珊瑚骨骼是如何以及何时进化的？从一个简单的水螅到一个复杂的葡萄牙军舰群之间的进化转变是什么？重建刺胞动物进化树对于我们理解伴随动物生命早期多样化的模式和过程非常重要。正式和非正式教育将从这项研究中产生。本科生、研究生和博士后学者，包括那些在科学领域代表性不足的群体的学者，将得到培训。研究人员将在一次全国科学会议上组织一次刺胞动物进化关系专题讨论会。将开发刺胞菌生命之树数据库，其中包括外联和学习内容，如图像、通用名称和密钥，旨在吸引各级学生以及潜水员和水族馆爱好者等感兴趣的用户。博物馆将根据研究结果进行展览，其中一个在堪萨斯大学自然历史博物馆展出，另一个名为“海洋中的刺细胞生物多样性”，将成为定于2008年在国家自然历史博物馆开放的新海洋馆的一部分。与后一个展览相结合的网站和教室海报（英语和西班牙语）将与由美国国家科学基金会教师研究经验项目支持的K-12教育工作者合作创建。