CAREER: A Bioanalytical System for Gene Expression Analysis on a Single Cell Basis

职业：基于单细胞的基因表达分析的生物分析系统

• 批准号: 0546012
• 负责人: Jeff Wang
• 金额: $ 40万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0546012&HistoricalAwards=false
• 关键词: CAREER; Bioanalytical; System; Gene; Expression

0546012WangThe objective of this proposal is to develop a platform to assess mRNA expression using a novel quantum dot FRET probe within a microfluidic environment. Characterization of the molecular probe and the microfluidic systems are proposed followed by a demonstration of the integrated system using a gene expression model. The education goals include BioMEMS course development, introducing a seminar series on Bio/Nano topics, undergraduate mentoring and an outreach component focused on involving high school students.

该提案的目的是开发一个平台，以评估mRNA的表达，在微流体环境中使用一种新的量子点FRET探针。分子探针和微流控系统的表征提出了随后的集成系统使用基因表达模型的演示。教育目标包括BioMEMS课程开发，介绍生物/纳米主题系列研讨会，本科生指导和重点涉及高中生的外展部分。