Collaborative Research: Integrated Microsystem for Ultrasensitive Airborne Pathogen Detection in Real Time

合作研究：实时超灵敏空气传播病原体检测的集成微系统

• 批准号: 0725528
• 负责人: Jeff Wang
• 金额: $ 14万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-11-01 至 2011-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0725528&HistoricalAwards=false
• 关键词: Collaborative; Research; Integrated; Microsystem; Ultrasensitive

Collaborative Research: Integrated Microsystem for Ultrasensitive Airborne Pathogen Detection in Real TimeAbstractThe objective of this research is to develop an airborne pathogen sensing system, capable of fast and sensitive environmental monitoring, for use in a broad range of infrastructures. The approach is to develop both microfluidic operations for sample collection and preparation and quantum dots-based nanosensors for identification of pathogens according to their ribosomal RNA sequences. The microfluidic unit and the nanosensor unit will be integrated into a system whose performance will be exemplified through analysis of Bacillus spores. The use of the droplet-based microfluidic operations will minimize the requirement of liquid volume and avoid the need for building moving components on the chip for sample processing that both reduces the consumption of biochemical reagents and the complexity in constructing an integrated microfluidic processing system. The highly sensitive rRNA nanosensors will eliminate the need for polymerase chain reaction (PCR) amplification, thereby simplifying assay protocols and system integration. This sensing system will have broader impacts with the societal benefits including (i) immediate detection of bio-warfare pathogens such as anthrax to prevent their possible catastrophe and (ii) surveillance of environments in, for example, transportation infrastructures, healthcare buildings, and workplaces. Besides, this research will also have significant impacts on education by (i) establishing micro/nano scientific visual library on web based on the research outcome; (ii) developing micro/nano science coursework with a lab session; and (iii) having undergraduates especially from the underrepresented groups to participate in the research activities.

协作研究：在实时提取本研究的目标的超敏感空气病原体检测的整合微型系统是开发一种能够在广泛的基础架构中用于快速和敏感的环境监测的机载病原体传感系统。该方法是开发用于样品收集和制备和基于量子点的纳米传感器的微流体操作，以根据其核糖体RNA序列鉴定病原体。微流体单元和纳米传感器单元将整合到一个系统中，该系统将通过分析芽孢杆菌孢子的分析来体现其性能。基于液滴的微流体操作的使用将最大程度地减少液体体积的需求，并避免在芯片上构建移动组件进行样品处理，以减少生化试剂的消耗量和构建集成的微流体加工系统的复杂性。高度敏感的RRNA纳米传感器将消除对聚合酶链反应（PCR）扩增的需求，从而简化了测定方案和系统整合。这种传感系统将对社会利益产生更大的影响，包括（i）立即检测生物 - 战线病原体（例如炭疽病），以防止其可能的灾难，以及（ii）对环境的监视，例如，运输基础设施，医疗保健建筑和工作场所。此外，这项研究还将通过（i）根据研究结果在网上建立微/纳米科学视觉库对教育产生重大影响； （ii）通过实验室会议开发微/纳米科学课程； （iii）本科生特别是来自代表性不足的群体参加研究活动的本科生。